腦外傷后細(xì)胞核因子κB（NF-κB）信號(hào)通路對(duì)自噬過(guò)程的調(diào)節(jié)及機(jī)制探討.pdf

1、蘇州大學(xué)碩士學(xué)位論文腦外傷后細(xì)胞核因子κB（NFκB）信號(hào)通路對(duì)自噬過(guò)程的調(diào)節(jié)及機(jī)制探討姓名：李生興申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：法醫(yī)學(xué)指導(dǎo)教師：陶陸陽(yáng)20080501T—heComributionofNuclearFac—tor—KBPath—wayto—AutophagyAncrTraumaticBrain塒uryinRatNF—KBActivatingUp—regualteAutophagicProcessafterTraumaticB

2、rainInjuryinRatAbstractObjection：ToinvestigatethecontributionofNFr。BpathwaytotheautophagicprogressionaftertraumaticbraininjuryinratmodelMethods：TraumaticbraininjurymodelwasinducedbyamodifiedweightdropdevicethatWasallowed

3、afleefalluntilthesiliconetip(diameter5mm)hittheexposedskullontheleftcerebralhemisphere，lmmlateraltothemidlineinthemidcoronalplaneSN50(10Itg)wasiIljectedintracerebroventricularly(icv)lOminbeforetheonsetofstrikeWesternblot

4、WasemployedtodeterminealternationsinBeelinl，Bcl2，cathepsinDandLC3inimpairedcDrtexandhippocampusatdifferenttimepointsafterTBI，aswellasparallelgroupsofmiceResults：TheexpressionofBeclinl，Bcl2，cathepsinDandLC3aresignificantl

5、yincreasedinimpairedcortexandhippocarnpusonlh3hm12h24hand48htimepohnafterTBIAteverypostinjurytime，Beclinl，cathcpsinDandLC3ⅡexpressioninSN50groupislowerwhileBcl2ishigherthanSalinegroupConclusions：1AutophagyWasactivatedbyT

6、BI2NF—vBpathwaycontributestotheregulationofautophagosomeafterTBI3Theexpressionoflysomalenzymecath印sinDWasaccommodatedbyNFr。BpathwayafterTBIKeyWords：Waumaticbrain’injury；autophagy；NFxB；Beclinl；Bcl2；cathepsinD；I，C3ⅡWritten