肌苷對腦缺血再灌注大鼠腦組織中COX-2、CaN、VEGF表達(dá)的影響及意義.pdf

1、青島大學(xué)碩士學(xué)位論文肌苷對腦缺血再灌注大鼠腦組織中COX2、CaN、VEGF表達(dá)的影響及意義姓名：李琴申請學(xué)位級別：碩士專業(yè)：神經(jīng)病學(xué)指導(dǎo)教師：郭云良20041128TheEffectAndSignificanceOfInosineOnExpressionOfCOX2，CaNAndVEGFFollowingCerebralIscbemieReperfuslonInRatsAB盯RACTobjective：Fromthechangesof

2、theexpressionofcyclooxygenase2(cox2)，calcineurin(CAN)andvascularendothelialgrowthfactor(vEGF)toobservethepossibleprotectivemechamismofInosineontheischemiccerebraltissue，andtoexploreiftheprotectiveeffectof／nosineisrelevan

3、ttotheexpressionofCOX2，CaNandVEGFaRerreperfusionofMCAOinratsMethods：ThemodeIsofmiddlecerebralarteryocclusionOⅥCAO)reperfusionwereestablishedwithSDmrs，whichweredividedintothecontrolledgroup(salinesolutionwasinjectedintrap

4、erisoneally)andtheInosinegroup(Inosinewasinjectedintraperisoneatly)Eachgroupwasdividedintoeightsubgroupsatreperfusion2h、6h、12h、24h、2d、3d、7d、14dwhichconsistedof4ratsInsituhybridizationandimmunohistochemical(echniqueswereu

5、sedtoinvestigatethedynamicchangesofCOX一2，CaNandVEGFincerebraltissueResults：①COX2mRNA：TheexpressionofCOX2mRNAoccurredat6h，peakedat12h，maintained24h～48hafterreperfusionanddecreasedgraduallytothelowestlevelat：4dThelevelofCO

6、X一2mRNAincerebralcortexwashigherthanthatinstriatumComparedwiththecontrolledgroup，theuseofInosinecouldelevatethelevelofCOX2’FhereWassignificantdifferencebetweenthetwogroups(P001)②COX一2：ThechangelIftheexpressionofCOX一2issi

7、milartothatofCOX2mRNA，butthepeal(ocowredat24hafterreperfusionanddecreasedrapidlyUptotherepcrfusion7d，itdroppedtothebaselineinbraintissueTheexpressionofCOX一2incerebralcortexWashigherthanthatinstriatanaComparedwiththecontr

8、olledgroup，injectingInosinecouldelevatethelevelofCOX，2Therewassignificantdifferencebetweenthetwogroups(P001)③CaN：TheexpressionofCaNincreasedtemporarilyat6h，12hand24hthendecreasedimmediatelyaflerMCAOAt7d，itdroppedtothebas