雌激素水平對三苯氧胺抑制乳腺癌細(xì)胞生長的影響及機(jī)制研究.pdf

1、華中科技大學(xué)碩士學(xué)位論文雌激素水平對三苯氧胺抑制乳腺癌細(xì)胞生長的影響及機(jī)制研究姓名：韓明強(qiáng)申請學(xué)位級別：碩士專業(yè)：普通外科學(xué)指導(dǎo)教師：黃韜20060401華中科技大學(xué)同濟(jì)醫(yī)學(xué)院2003級碩：E畢業(yè)論文StudyofEffectsofDifferentLevelsofEstradiolonInhibitionofTamoxifenonHumanMammaryCancerCells(ER)inVitroBreastandThyroidSur

2、geryCenterUnionHospita‘ron百imedicalcollegeHuazhongUniversityofScienceandTechnologyPostgraduate：HANMingqiangAbstract：ObjectiveTostudytheeffectsofdifferentlevelsestradioloninhibitionoftamoxifenonhumanmammarycancercells(ER)

3、invitroMethodsEstrogenreceptor(ER)expressionwasobservedbyimmunohistochemicalmethodsEstrogenreceptor①R)positiveMCF一7humanbreastcancelcelllinewastreatedbythesameleveloftamoxifenanddifferentlevelsofestradiolinvitroCellproli

4、ferationwasevaluatedbyMITassayThecellcycleofMCF一7cellswasmonitoredbyflowcytometryResultsERwaspositiveinMCF7cells／／2atconcentrationsbetween110’12mol／Lto110。mol／LsignificantlystimulatedthegrowthofMCF一7TAM(110。mol／L)inhibit

5、edthegrowthofMCF一7significantlyE2atdifferentlevelsmayinfluenceinhibitoryoftamxifenonMCF7celllinesE2(110—9moVE)makesinhibitionoftamoxifenonMCF7celllinesvaluelessIntheblankgroup，proportionalityofcellsarrestedatG0／G1phasewa

6、s67％，andthatwas7973％intheonlyTAMgroupHoweverE2n10—9mol／L)madeitbecome5833％andmadeproportionalityofcellsatSphase2382％，toitspeakConclusionE2istheriskfactorofbreastcancerandtheconcentrationaroundbreastcancercellsmayinfluenc