臍血單個(gè)核細(xì)胞Toll樣受體4在子癇前期中的表達(dá)研究.pdf

1、華中科技大學(xué)碩士學(xué)位論文臍血單個(gè)核細(xì)胞Toll樣受體4在子癇前期中的表達(dá)研究姓名：徐丹芬申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：婦產(chǎn)科學(xué)指導(dǎo)教師：吳超英201005華中科技大學(xué)碩士學(xué)位論文華中科技大學(xué)碩士學(xué)位論文3TheexpressionofTolllikerecept4ofneonatalcdbloodmononuclearcellsinpreeclampsiaMasterCidate:XuDanfenSupervis:Prof.WuChaoyin

2、gAbstractObjective:ToinvestigatetheexpressionofTLR4inneonatalcdarterialbloodmononuclearcellsTNFαintheserumthustoexplethemechanismofTLR4inpreeclampsia.Methods:Twentypatientssufferingfrompreeclampsiatwentynmalpregnancypati

3、entsweredividedintotwogroups:experimentalgroupcontrolgroup.SeparatingneonatalcdarterialbloodmononuclearcellstheTLR4mRNAproteinexpressionweremeasuredbyrealtimequantitivePCRWesternBlotmethodtheexpressionofTNFαintheserumwas

4、detectedbyELISA.Results:TheTLR4mRNAlevelinneonatalcdarterialbloodmononuclearcells(2ΔCT:0.06690.0220）TLR4proteinexpression(ODratio:0.810.16%)TNFαlevelintheserum（9.744pgml1.362pgml）wereallincreasedinexperimentalgroupcompar

5、edwiththecontrolgroupthedifferenceswerestatisticallysignificant(P0.05).TheexpressionofTLR4mRNATNFαinexperimentalgroupcontrolgroupshowedpositivecrelation，thecrelationcoefficientswere0.520.53respectively(P0.05).Conclusion:

6、TheexpressionofTLR4inneonatalcdarterialbloodmononuclearcellsTNFαintheserumwereallincreasedintheexperimentalgroup.TheexpressionofTLR4TNFαinexperimentalgroupcontrolgroupshowedpositivecrelation.TLR4mediatedreleaseofinflamma