聯合干預基因CyclinD1和Bcl-xL對非小細胞肺癌細胞增殖和凋亡的影響.pdf

1、華中科技大學碩士學位論文聯合干預基因CyclinD1和BclxL對非小細胞肺癌細胞增殖和凋亡的影響姓名：陳影申請學位級別：碩士專業(yè)：內科學（呼吸內科）指導教師：熊維寧201105華中科技大學碩士學位論文華中科技大學碩士學位論文3CombinedCyclinD1BclxLgenetherapyattenuatedcellgrowthpromotedcellapoptosisinNonsmallcelllungcancerDepartmen

2、tofrespiratyTongjiHospitalTongjiMedicalCollegeHuazhongUniversityofScienceTechnologyInstruct:Prof.XiongWeiningPostgraduate:ChenYingABSTRACTObjectives:TodeterminecombinedCyclinD1BclxL’spotentialfeasibilityasatherapeutictar

3、getweconstructedarecombinantplaspcDNA6.2GWEmGFPmiRthatexpressedaCMVpromoterdrivenmicrNAtargetingCyclinD1(CyclinD1miRNA)BclxL(BclxLmiRNA)thesetwogenes(CyclinD1BclxLmiRNA).Thenthevect’sabilitytoinduceRNAinterferenceinvitro

4、alterapoptosisinductioninlungcancerwasassessed.Methods:Fourgroupswasused:thegroupofblankcontrol(untreatedcells)CyclinD1miRNABclxLmiRNACyclinD1BclxLmiRNA(transfectedcells).ThecelllineswereusedA549NCIH441.ThelevelsofmRNApr

5、oteinofBclxLCylinD1weredetectedbyRTPCRWesternblot.Apoptosisproliferationoflungcancercellswereevaluatedbydimethylthiazoldiphenyltetrazoliumbroe(MTT)cellcountflowcytometry.Results:Ourresultssuggestedthattherecombinantplasp