誘導分化降低膠質(zhì)瘤干細胞樣細胞表型及其致瘤性.pdf

1、華中科技大學碩士學位論文誘導分化降低膠質(zhì)瘤干細胞樣細胞表型及其致瘤性姓名：胡峰申請學位級別：碩士專業(yè)：神經(jīng)外科指導教師：雷霆201105華中科技大學碩士學位論文華中科技大學碩士學位論文2InduceddifferentiationsuppressesthestemcellppertiestumigenesisofstemlikegliomacellsPostgraduatestudent:HuFengSupervis:Prof.LeiT

2、ingDepartmentofNeurosurgeryTongjiHospitalTongjiMedicalCollegeHuazhongUniversityofScienceTechnologyAbstractObjectiveUseATRAtoinducestemlikegliomacellstodifferentiatecomparestemlikecellspropertiesbiologicacteristicsbetween

3、stemlikegliomacellsincludingC6celllinesprimarygliomacellstheirdifferentiatedcells.MethodsGliomacellswereculturedwithneurospherefmationassayinserumfreesupplementedmedium.Stemlikegliomacells(SLGC)weredifferentiatedwithallt

4、ransretinoicacid(ATRA)containingmedium.Growthacteristicsincludingmphologicaldifferencescapacityofselfrenewalweredetectedcontrastivelythroughneurospherefmationassay.Expressionsofstemcelldifferentiatedcellmarkersweredeterm

5、inedbyreversetranionpolymerasechainreaction(RTPCR)WesternBlottingFlowCytometry(FCM).SLGCdifferentiatedcellswereimplantedstereotacticallyintracraniallyintotheBalbcnudemicetocontrastthecapabilityoftuminitiationthetummodels

6、weredetectedbyhematoxylineosin(HE)staining.AlldataanalyseswereperfmedwiththeSPSSsoftwareversion17.0.ResultsOurdatasuggestthatinvitrodifferentiationofSLGCswithATRAinducesthegrowthacteristicschangesofgliomacellsdownregulat