指紋圖譜結(jié)合化學(xué)計量學(xué)在中藥質(zhì)量控制中的研究.pdf

1、南昌大學(xué)碩士學(xué)位論文指紋圖譜結(jié)合化學(xué)計量學(xué)在中藥質(zhì)量控制中的研究姓名：袁亞洲申請學(xué)位級別：碩士專業(yè)：分析化學(xué)指導(dǎo)教師：倪永年20081120ABSTRACTABSTRACTWiththerapiddevelopmentofhyphenatedinstrumentsandrelatedchemometricsmethods，itispossibletoprovidepowerfultoolsandextensiveprospectfort

2、hebuildingandevaluatingofTraditionalChineseMedicine(TCM)fingerprintMoreovermultivariatechiveresolutionmethodsarcappliedinresolutionofthetwodimensionaldataobtainedbyhighpeIformanceliquidchromatographywithgaschromatography

3、massandfouriertransforminfrared；andpatternrecognitionmethodsareemployedtoestablishandresolvethefingerprintsoftraditionalChinesemedicine，whichcouldbeusedforthequalitycontrolofChinesemedicineInChapterone，areviewonresearchs

4、tatusanddevelopmentforegroundwasarised，whichtheconception，characteristics，researchcontentanditsimportanceforqualitycontrolofTCMwereintrodcedmainlyInaddition，themethodsappliedforfingerprintanalysisofTCMwasalsoreviwed，andt

5、hechemometricsmethodswerediscussedspeciallyThetrendoffingerprintanalysiswasmentionedintheendInChaptertwo，chromatographyfingerprintsof45FoliumPerillaesampleswereobtainedbyHPLC—DADandGC／MSThesesamplesWerecollectedfromfivep

6、rovincesinChinaWiththeapplicationofprincipalcomponentanalysisfPCA)，itwasfoundthat，HPLCfingerprintscouldbeusedtoexplainFoliumPerillaesamplesorigins，andtheGC／MSdatacouldbeusedtoexplaineditscollectedtimesandvaritiesThepropo

7、sedmethodcouldbesuccessfullyappliedtoclassifytheFoliumPerillaesamplesintodifferentgroupsthatcorrelatedtoitscollectedtimesandvarities，anditcouldbeusedfotthequalitycontrolofFoliumPerillaeeffectivelyMoreovertwosupervisedpat

8、ternrecognitionalgorithmsWereappliedtoprocessedthedata，andsimilarresultscouldbeobtainedInchapterthree，highperformanceliquidchromatographyandFouriertransforminfraredfingerprintsofHanglianshangqingpillswereestablishedfor7o

9、f26samplesItwasfound廿1atgeneticalgorithmwasapplicationforfeatureselectioninFouriertransforminfraredspectradatasets，anditcouldselectedthevariableseffectivelyTheresultsbasedonprinciplecomponentanalysisshowedthattherewereob

10、viousdifferencesamongtheHanglianshangqingpillsproducesbydifferentmanufacturesTheseobservationsfromthePCAbiplotweresupportedbyhierarchicalclusteranalysis，andthemethoddevelopedcanbeeffectivelyusedtocontrolthequalityoftradi

11、tionalChinesemedicineHanglianshangqingpillsInchapterfourhighperformanceliquidchromatographyanddiodearraydetector(HPLCDAD)wasemployedtoestablishthefingerprintofRadixAngelicaeDahuricae(RAD)Twationalchemometricsmethodswerea

12、ppliedtesolvethehighperformanceliquidchromatographicfingerprintofRADPrincipalcomponentanalysis(PCA)andclusteranalysis(CA)wereemployedtoresearchthesimilaritiesanddissimilaritiesamongthe30RAsamplesBothofthetwopatternrecogn

13、ition(PR)methodscouldbesuccessfullyusedtoclassifytheseobjectsamplesrespecttotheirsourcesThemultipleresultsmanifestedthatthereferredtechnologiescanbeefficientlyusedtoelassifytraditionalChinesemedicine(TCM)RADintogroups，wh

14、ichcorrelatedtotheiractivecomponents，andprovidingareferencemethodforchemicalpattemrecognitionofotherTCMKeywords：fingerprint；highperformanceliquidchromatography；Fouriertransforminfrared；gaschromatographymass；chemometrics；