胚胎提取物誘導(dǎo)體外培養(yǎng)腫瘤細(xì)胞凋亡的實驗研究.pdf

1、東北師范大學(xué)碩士學(xué)位論文胚胎提取物誘導(dǎo)體外培養(yǎng)腫瘤細(xì)胞凋亡的實驗研究姓名：金青云申請學(xué)位級別：碩士專業(yè)：生理學(xué)指導(dǎo)教師：胡健20080601AbstractMaligIIanttumoranobstinateillnessthreateninghealthandlife，ismainlycuredbythechemicaltfeatment，althoughresearcherhasmade孕eatpro鱸essintreatingit

2、BecausepresentchemicalmedicinesalwayscausesigIlificantsecondaryeffccts，newantitumordrugsarc伊eatlynecessar)，tobedeVelOpedReccntoncotherapybelieVesthat：thetumorSaretheIiVecenmasswhichshouldhaVeoccu玎edapoptosisApoptosisisap

3、ro伊ammaticdeathp脫essiIlcelldeVelopmentIIlotherwords，jtcanals0beregarded瑟粕otherfb皿ofcelldjfferentjation，whichsi鄹ificantlyc011relateswithitsapoptosisintheearlyembr)，Onicdcvelopment(bncemingabouttheexistencco“hefacto硌induci

4、ngthedifferentiation弛dapoptosisinthea_bovestage撲dtheinlportantfunctionoftheapoptosisinantitumorprolce豁，rese刪Iergraduallypayattentiontothcearly—embryonicdeVelopmentalfa咖璐，theirinducingtumorccIlapoptosis鋤dapplicationinanti

5、tumortI，eatmentThisthesismainlyf＆usesondeVelopingnewmethodt0stilllulate0rinducetheapoptosis0fthetumorccllsWeadoptedthecarlyembryonicfhct0璐extractedfmmthechicken觚dIjrogembryos，whichweredirectlyappliedtotheHelaaIldHepG2ceI

6、lsinVitr0Througllthemorpholo畫cal0bservation鋤ddetectionViaflowcytomet彤we卸alyzedthe礎(chǔ)iVity0fthefactorsinthedif!ferentiation鋤dapOptosis0ftheab0VetumorcellsItw弱demonstratedbythemorpholo舀calobservationthataftertheapplicationof

7、theextracts，the星互0w啦of維etumofceHswereres|rictedandshowedtypica王apop幻sisphenomen冊：fec0Vefy’growingrounding，exfoliation，suspension，dcCfe鶴eofmicrovilli，breakdownofthecllromosomes，VacuolizationofmembranesandapoptoticbodyThef

8、lowcytometicaldetectionalsoproVedthatextractsf沁mthechickembryocouldinducesignificantapoptosisofHelacellsConcentrationof12mg，mlinducedapoptoticrateof70％TlllisthesistentatiVelyconfinnedthattheearIyembryonicextractscouIdind