乳腺癌骨髓、血液微轉(zhuǎn)移及其手術(shù)、化療后變化的動態(tài)研究.pdf

1、第三軍醫(yī)大學(xué)碩士學(xué)位論文乳腺癌骨髓、血液微轉(zhuǎn)移及其手術(shù)、化療后變化的動態(tài)研究姓名：張帆申請學(xué)位級別：碩士專業(yè)：外科學(xué)（普通外科）指導(dǎo)教師：姜軍20030501墨三堊墾盔堂堡主堂焦笙苧MicrometastasisofbonemarrowandbloodanditschangesafteroperationandchemotherapyinbreastcarcinomaAbstract0bjective：Breastcarcinomais

2、oneofthecommonestmalignanttumorsofwomenFindingoutthemetastasisearlyisimportantforthetumorstaging，prognosisjudgmentandtherapeuticmethoddeterminationinthosepatientswithbreastcarcinomaSomeinvestigationshaveprovedthatmicrome

3、tastasisofaxillarylymphnodeisanindependentprognosisfactorinbreastcarcinomaTherehavebeenfewstudiesforthedynamicchangesonmicrometastasisofbonemarrowandperipheralbloodInthisstudy，thechangesofmicrometastasisinbonemarrowandpe

4、ripheralbloodweredetected，andsomeregularpatternsofmicrometastasisanditseffectofoperationandofpostoperativechemotherapyweresurveyedinthepatientswithbreastcarcinomaStudyingthemicrometastasisofbonemarrowandbloodcouldbehelpf

5、ulfortheearlydiagnosisofdistantmetastasisanddeterminationthetreatmentinbreastcarcinomaMethods：ThesamplesofperipheralbloodandbonemarrowweredetectedwithCK一19mRNAbyRT—PCRfor40casesofbreastcarcinomabeforeoperationAndtheexpre

6、ssionchangeofCK一19mRNAinperipheralbloodwasdetectedontheseconddayafteroperationandonthelastdayofthefirstcourseofpostoperativechemotherapyThesamplesofperipheralbloodandbonemarrowweredetectedin10casesofbenignbreastdiseaseas

7、thecontrolgroupwiththesamemethodReSUIts：1ThepositiverateofCK一19mRNAexpressioninbonemarrowwas2250％(9／40)，ThepositiveratesofCK一19mRNAexpressioninthepatientswith0phaseandIphase，IIphase，IIIphasewere0％(0／6)，1600％(4／25)，5556％(

8、5／9)respectivelyTherewereremarkablesignificanceofthedifferenceinthepositiveratesbetweenthedifferentphases(P005)ThepositiveratesofCK一19mRNAexpressionininfiltratingductalcarcinomaandinfiltratinglobularcarcinomawere2692％and