家蠅抗菌肽生產及制劑研究.pdf

1、湖南農業(yè)大學碩士學位論文家蠅抗菌肽生產及制劑研究姓名：鄒鋒申請學位級別：碩士專業(yè)：臨床獸醫(yī)學指導教師：肖兵南20070501ABSTRACTMnscaDomesticandLarvaeisagoodresourcesoffeedresolffcesandmedicineresourcesbecauseofitsbroaddistributioneasycultivationandlowcostWestartedourworkinorde

2、rtofindnewactiveantimicrobialpeptidesWeestablishingcultivationmethodforMuseadomesticaandlarvae，findinganewfeedingmethodformuscadomesticawithannimalmamlreE自fectsofenvironmentalfactorsonmassreproductionofMnscaDomesticLarva

3、eWaSmadeinlaboratorytheresultsshowedthattheoptimumtemperatureofadultovipositionandl&rvaegrowthw鷸25℃TheoptimumwatercontentoflalwadietWaS70％Thefactorsaffectinglarvaweightwerediet，temperatureandbreedingdensityindescendingor

4、derAndthenWeoptimizetheantibacterialprptidesproducedtechnicsⅥredetectitcaninhibittheresistantbacterialgrowththeMICandMBCoftheanmicrobialpeptidefromtheMIlScadomesticlarvaewaSdeterminedandtheyCaninhibitthegrowthofdifferent

5、speciesofmultidrugresistantGrampositiveandmultidrugresistantGramnegativebacteriatodifferentextentFinallywedeterminetheeffectofoHandternperatureOllstabilityofantibacterialpeptideBytheorthogonaldesignL16(45)，wedeterminedth

6、eprescriptionsofantimicrobialpeptidegel：antibacterialpeptide(259)；bases(39)；Slllfaceactiveagents(019)；hnmeetant(209)；water(1009)；theoptimumpHWaS45Theinhibititionofantimicrobialpeptidesprayandgeltesistantbacteriaaremeansu

7、redWeconcludethesprayandgelarepotentialtoclinicnseFinallytheclinicale伍eacyandtheclinicalindicationsofMuscaDomesticantimierobialpeptidegelWglgobservedresultingthattheaveragecureande舒cientpercentageofthisantibactriapeptide

8、gelforSevereclinicalmastitiswere60％and85％；andtheaverageCUreandefficientpercentageof3daysadministratedthisgelforsubclinicalmastitiswere74％and88％and90％and94％for7daysadministratedKeyWords：MuscaDomestic；Antimicrobiaipeptide；