黑脊倒刺鲃胚胎、幼魚的發(fā)育及原生殖細(xì)胞的起源、遷移和分化的研究.pdf

1、福建師范大學(xué)碩士學(xué)位論文黑脊倒刺鲃胚胎、幼魚的發(fā)育及原生殖細(xì)胞的起源、遷移和分化的研究姓名：蘇敏申請學(xué)位級別：碩士專業(yè)：動物學(xué)指導(dǎo)教師：尤永隆林丹軍2003.4.1AB—STRACTInthispapertheembryonicandthelarvae’sdevelopmentofteleost，SpinibarbusCaldwellianditsPGCs’origin、migrationanddifferentiationwerest

2、udiedatmicroscopicandsubmicroscopiclevelsnlefertilizedeggsofSpinibarbusCaldwelliwereobtainedbyartificialinseminationBasedonthemorphologicalfeaturesanddevelopmentalcharacteristicsoftheembryo，theprocessofembryonicdevelopme

3、ntcouldbedividedinto22stageswhichbelongedto6embryonicperiodsAtthewatertemperatureof25—286C，theembryonicdevelopmentlasted45hoursand15minutesAndtheprocessofthelarvae’sdevelopmentcouldbedividedintofourstagesIncomparisonwith

4、somaticcells，PGCswerelarge謝tlllargenuclei，whichcontainedoneortwoprominentnucleoliandpossessedadistinctnuclearmembraneThecytoplasmofPGCswasweak—acidophilicInaddition，therewereannulatelamellaeandplentyofmitochondriainthecy

5、toplasmUltrastructuralanalysisofPGCshadrevealedthatthepresenceoflotsof“electron—densebodies”whichappearedashigh—electron—densecytoplasmicinclusionsinthevicinityofnuclearmembrane，oftenobservedinassociationwithmitochondria

6、TheseelectrondensebodieswerestrongalkalophilicDuringthemigration，thedeformedPGCswhichwerelongerandslimmerwithanextendednuclearwereobservedBlastulastagewastheearlieststageatwhichPGCswasobservedPGCswereassociatedwiththebla

7、stodermmarginatfirstandsubsequentlyremainedinthehypoblastoftheembryonicshieldregionduringmid—gastrulaandneurulastageLatertheymigratedtothesplanchnicmesodermduringthestageofmusculareffectandthenproceededaroundtheguttothed

8、orsalmesenteryduringhatchingstageIntheend，theytranslocatedtothegenitalridgeswheretheyformedtheindifferentgonadwiththesomaticcellsofgenitalridgesThewholemigrationprocesshadsomethingtodowithboththesurroundingtissuesandthea