CEM15基因的生物信息學分析.pdf

1、分類號密級UDC學位論文CEM15基因的生物信息學分析基因的生物信息學分析（題名和副題名）王翊（作者姓名）指導教師姓名馬義才馬義才副教授副教授電子科技大學電子科技大學成都成都（職務(wù)、職稱、學位、單位名稱及地址）申請學位級別碩士碩士專業(yè)名稱生物醫(yī)學工程生物醫(yī)學工程論文提交日期2005.05論文答辯日期2005.06學位授予單位和日期電子科技大學電子科技大學答辯委員會主席堯德中堯德中教授評閱人2003年1月13日注1注明《國際十進分類法UD

2、C》的類號CEM15基因的生物信息學分析IIAbstractRecentlytheresearchonVifhasrevealedanovelcomponentofinnateimmunesystemAPOBEC3G（CEM15）whichisacytidinedeaminaselethallyhypermutatesretrovirusesretroelements.APOBEC3GthatbelongstoAPOBECfamilya

3、ctsinDNAeditingtoblockthereplicationofretrovirusessuchasHIV1.InfactAPOBEC3GisabletodefenseawidespectrumofdistantlyrelatedretrovirusesinterfereswithHBVthroughadifferentmechanismfromHIV.APOBEC3G（CEM15）isacellularproteinreq

4、uiredfresistancetoinfectionbyhumanimmunodeficiencyvirustype1hasbeenshowntobeacytidinedeaminasewhichisincpatedintovirionssubsequentlytriggersmassivedeaminationofdeoxycytidinetodeoxyuridinewithintheretroviralminusstrcDNA.V

5、ifwhichisaminproteinencodedbyHIV1oftenplaysanimptantroleinviralreplicationofHIV1.OnlyinthevirusproducingcellVifexpressionisnecessaryinitsabsenceinfectionofasubsequenttargetcellterminatesatapostentrystepthroughtheactionof

6、aninnateantiviralmechanism.Vifproteinhastwodomains:onebindstoAPOBEC3GtheotherregulatesthedegradationofAPOBEC3GbyaconservedsequenceSLQ(YF)LAmotif.VifproteininducesrapiddegradationofAPOBEC3Gbyubiquitinationwhichisaproteoso

7、medependentpathway.AsCEM15maybeanewwaytocureAIDSsoitisveryimptanttopeople.Butwenowknowlittleaboutitweshouldanalyzeitcarefully.Inthispaperwedothisbybioinfmaticsways.ThegenewasanalyzedfPIMwAminoacidcontentHyhobicicyprotein

8、secondarystructuremotifdomainsuperfamilyelectronicexpressionpatternPositiononChromosomebybioinfmaticsmethods.Theiginalityofthedissertationisasfollows:1)ThedissertationreviewstheresearchofCEM15(APOBEC3G)whichwaythisgenema