γ-氨基丁酸受體與小鼠卵母細(xì)胞成熟度的相關(guān)性研究.pdf

1、分類號：R711.59密級：不保密UDC：610學(xué)校代碼：11065碩士學(xué)位論文γ氨基丁酸氨基丁酸受體受體與小鼠卵母細(xì)胞成熟度的相關(guān)性研究與小鼠卵母細(xì)胞成熟度的相關(guān)性研究戴皇冠指導(dǎo)教師郝翠芳教授學(xué)科專業(yè)名稱婦產(chǎn)科學(xué)論文答辯日期2016年5月28日AbstractObjectiveInthisexperimentweinvestigatedtheexpressionofγaminobutyricacid(GABA)Areceptsubun

2、itsinoocytesitssurroundingcumuluscellsofthemouseexpledtheroleofduringovarianfoliculardevelopmentoocytematuration.MethodsImmaturefemaleKunmingmiceweretreatedwithPregnantMareSerumGonadotropin(PMSG)toinducethefolliculardeve

3、lopmentwithPMSGhumanChionicGonadotropin(hCG)topromotetheovulation.FirstwemeasuredthetranomiclevelofGABAAreceptsubunitsinoocytesitssurroundingcumuluscellsbyqRTPCR.SecondWesternblottingImmunofluescencetechniquewereusedtode

4、tecttheexpressionoftheα5incumuluscellsisolatedfromoocytesaccdingtooocytenuclearmaturity.TheexpressionlocalizationofGABAinthegerminal(GV)vesiclemetaphaseⅡ(MⅡ)cumuluscellssurroudingoocyteswereevaluatedbyimmunohistochemical

5、analysis.ResultsGABAAreceptsubunitshavedifferenttranionallevelinmousecumuluscellsatdifferentnuclearmaturationstage（GVMⅡ）.ComparingtheCCsisolatedfromoocytesatGV(CCGV)withtheMⅡgroups(CCMⅡ)wefoundtheexpressionlevelsofα5β3we

6、reupregulatedintheCCMⅡgroups（P0.05）.ButtherelativemRNAabundanceofα3δπweredownregulatedintheCCGVgroupscomparedtointhoseCCMⅡgroupsθexpressionlevelexhibitednosignificantdifferenceinCCGVCCMⅡgroups（P0.05）.Thetranionallevelofα

7、4mRNAisabsentintheCCMⅡgroupbutitsmRNAispresentinCCGVgroup.ByWesternblottingImmunofluescencetechniqueα5werehighexpressedintheMⅡcumuluscellssurroudingoocytesthaninthoseatGVstage（P0.05）.TheGABAwasobservedtoexpressintheGVMⅡc

8、umuluscellsfrommouseoocytesbyimmunohistochemicalanalysis.ConclusionWefoundthatGABAAreceptsubunitsexhibiteddifferentialtranionallevelsinmousecumuluscellsatdifferentoocytenuclearmaturitystages.Theexpressionofγaminobutyrica

9、cidAreceptsubunitsincumuluscellsofthemouseindicatesthatGABAAreceptsubunitsmayparticipateintheregulationofovarianfolliculardevelopmentoocytematuration.Postgraduatestudent:HuangguanDai(ObstericsGynecology)DirectedbyProf:Cu