白刺黃酮的提取分離及有效部位抗氧化作用的研究.pdf

1、石河子大學(xué)碩士學(xué)位論文白刺黃酮的提取分離及有效部位抗氧化作用的研究姓名：樊蓮蓮申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：藥理學(xué)指導(dǎo)教師：劉金榮20070501白剌黃酮的提取分離與有效部位抗氧化作用的研究Studytheextraction、purification、separationofflavonidsandantioxidativeeffectoneffectivefractionsfromNitrariaMasterpostgraduate：Fa

2、nLianlian(Pharmacology)Directedby：ProfessorLiuJingrongAbstractQ燃ThistopicispurposethatoptimalextractionconditionoftotalflavonoidsinfruitofNicaeaisdeterminedScreeningcontentofflavonoidsindifferemmaturetimeoffruitofNitrari

3、aanditsleafAbstractiontotalflavonoidofNitrariaidentificationmooomasofabstractiontheethanolExtractionoffndtofNitrariaisevaluatadOilregulatinglipidandincreasingantioxidativeabilityMeanwhiletotalflavonoidsandfruitoilisevalu

4、atedonincreasingantioxidativeabilityMethods：determinationoftotalflavonoidsinfruitofNitrariabyUVspectrophotomcterSinglefactorexperimemwasconductedtooptimizetheextractionprocessoftotalflavonoidsinfruitofNitrariaTheinfluenc

5、eofeachfactorWaSmeasuredinorthogonalexperimentComparewithcontentoftotalflavonoidsbetweenindifferenttimefruitsofNitrariaanditsleafbyoptimizetheextractionprocessAbstractiontoudflavonoidofNitrariabyPolyamideandscphadexcolum

6、nchromatography]identificationbyPolyamidefilm；monomersofabstractionweredeterminedbyinfraredray、nuclearmagnetic“箔Onan∞andmassspectrometrytoanalyzeReproductionmodelofhighlipid，inordertoevaluateabilityofregulatinglipidandin

7、creasingantioxidativeintheethanolExtractionoffruitofNitrariaReproductionmodelofsubacotengmgmiceinordertoevacuateabilityofincreasingantioxidativeintotalflavonoidsandfruitonofNitraria—R—e—su—l—t—：Theconcentrationofethanol、

8、temperature、numberoftimes、w／vandtimesareeffectingextractionofflavonids：contentoftotalflavonoidsofmaturefruitisrelativelyhigh；theethanolExtractionoffrtdtofNitraria、totalflavonoidandthefruitoilswcreeffecteddifferentguideli

9、ne，Thedegenerationofhepaticfiaty伽bemodifiedTwomonolneiswereobtainedthrougholyamideandsephadexcolumnchromatographyConc1usio—n：111“highestextractionrateis75％etinmol、85“C、h7(w／v)andextracting4times(2hforeuchtime)2contentoft

10、otalflavonoidsmaturefruitofNitrariaisrelativelyhi咖3monomersofNitrar／aKaempferolandQuercetinincreaseantioxidativeabilityfromreferences4theethanolExtractionoffruitofNitrariahasasatisfactoryeffectonregulatinglipidandincreas