GP-7對人舌鱗癌Tca-8113細胞體外轉(zhuǎn)移潛能的影響.pdf

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4、 II Effects of GP-7 on the metastasis potential of Human tongue carcinoma Tca-8113 cells in vitro Abstract Objective: 4-[4″-(2″, 2″, 6″, 6″-tetramethyl-1″- piperidinyloxy nitroxide radical) amino] -4′-demethyl epipodopHy

5、llotoxin (GP-7) is one of semi-synthesized derivatives of nitroxide-introduced pHyllotoxin, which has lower toxicity and higher activity. It has been reported that GP-7 has significant anti-tumor effects on many kinds of

6、 cancer. However, the effects and mechanisms of GP-7 on Tca-8113 cells’ proliferation, invasion, migration and adhesion viabilities have not been reported until now. In the study, we investigated the effects and mechanis

7、m of GP-7 on Tca-8113 cells proliferation and metastasis in vitro. Methods: The proliferation viability of Tca-8113 cells treated with GP-7 was measured using Sulforhodamine B (SRB) assay. The viabilities of Tca-8113 cel

8、l’s invasion and migration were evaluated by Transwell chamber migration assay. The viability of Tca-8113 cell’s adhesion was assayed by cell-matrix adhesion assay. The protein expression levels were determined by Wester

9、n blotting analysis. The MMP-2 and MMP-9 levels were determined by Gelatin ZymograpHy assay. The NF-κB P65 nuclear transferation and activation capacity were determined using Nuclear Translocation Assay Kit. Results: The

10、 SRB assay showed that GP-7 25μg/mL significantly inhibited Tca-8113 cell proliferation. The inhibition rates of GP-7 in 24h, 48h, and 72h respectively were 67.49%, 70.58%, and 94.40%, respectively. GP-7 25μg/mL obviousl

11、y inhibited Tca-8113 cell invasion, migration and adhesion in 24h, respectively and their inhibition rates were 84.89%, 79.16%, 58.69%, respectively. Moreover, the expressions of p-Akt and p-IKK were reduced after treate