擬南芥MIR858及其靶基因AtMYB20相關(guān)轉(zhuǎn)基因植株的構(gòu)建及初步定位分析.pdf

1、南京農(nóng)業(yè)大學(xué)碩士學(xué)位論文擬地芥MIR858及其靶基因AtMYB20相關(guān)轉(zhuǎn)基因植株的構(gòu)建及初步定位分析姓名：劉飛申請學(xué)位級別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：楊志敏201105ABSTRACTCoNSTRUCTIoNoFTRANSGENIC4R4腳D|f，S磚EXPRESSINGl殂R858ANDITSTARGET彳刀玎凇2汐，ALONGWITHGUSLOCATIoNANALYSISABSTRACTnm斟Asareendogeno

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5、re曲∞s證幽遺ea35s：：MIR858，35s：：m5mM髓20，35s：：mM髓20，a試MIM858The∞conS訊Ictssuccessmllywere缸amfomedint0Arabidopsispl鋤峪via彳gm6口cfP，f搠mediated缸a璐forma士ionpr0協(xié)c01T10tbisend，T2plantShVebeenob缸lilledInadditio鞏wecom口uctedm緞甜8jG嬲and州嘶瑚2

6、DG潞眥sg砌cpl鋤tSUsing廿1eseGUSr印ongenes，wecouldident毋nlelocationofb0廿llniR858鋤dAtMYB20OuranalysisshowSt11at尬R8粥孤d爿f腳B加c孤beexpressedinvarioustissuesiIlcludhlgVei瑪pod，shoots，rootsandotllersTheseresultsiIldicatct11atmiR858甜ld刪Y