2023年全國碩士研究生考試考研英語一試題真題(含答案詳解+作文范文)_第1頁
已閱讀1頁,還剩107頁未讀 繼續(xù)免費閱讀

下載本文檔

版權(quán)說明:本文檔由用戶提供并上傳,收益歸屬內(nèi)容提供方,若內(nèi)容存在侵權(quán),請進行舉報或認領(lǐng)

文檔簡介

1、Novagen?DERING8005267319?TECHNICALSUPPT8002070144AGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGAGACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCTAGCATGACTGGTGGACAGCAAATGGGTCGCGGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTG

2、AGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAMetAlaSerMetThrGlyGlyGlnGlnMetGlyArgGlySerGlyCysEndpET3b...GGTCGGGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAA...GlyArgAspProAlaAlaAsnLysAlaArgLysGluAlaGlu

3、LeuAlaAlaAlaThrAlaGluGlnEnd...TACCATGGCTAGC...pET3cd...GGTCGGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAMetAlaSer......GlyArgIleArgLeuLeuThrLysProGluArgLysLeuSerTrpLeuLeuProProLeuSerAsnAsnEndC

4、CCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGNcoIpET3dNdeIT7?TagT7terminatBamHIBpu1102IT7terminatprimer#693373pET3arbsBglIIXbaIT7promoterprimer#693483T7promoterThepET3advectscarryanNterminalT7?TagsequenceBamHIcloningsite.Thesev

5、ectsaretheprecursstomanypETfamilyvectsthepET23ad()seriescrespondstopET3adbutincpatesseveraladditionalfeatures.Uniquesitesareshownonthecirclemap.NotethatthesequenceisnumberedbythepBR322conventionsotheT7expressionregionisr

6、eversedonthecircularmap.ThecloningexpressionregionofthecodingstrtranscribedbyT7RNApolymeraseisshownbelow.pET3asequencelmarksT7promoter615631T7tranionstart614T7?Tagcodingsequence519551T7terminat404450pBR322igin2814blacodi

7、ngsequence35754432ThemapsfpET3bpET3cpET3darethesameaspET3a(shown)withthefollowingexceptions:pET3bisa4639bpplassubtract1bpfromeachsitebeyondBamHIat510.pET3cisa4638bpplassubtract2bpfromeachsitebeyondBamHIat510.pET3disa4637

8、bpplastheBamHIsiteisinthesamereadingframeasinpET3c.AnNcoIsiteissubstitutedftheNdeIsitewitha1bpdeletionatposition550ofpET3c.AsaresultNcoIcutspET3dat546.Ftherestofthesitessubtract3bpfromeachsitebeyondposition551inpET3a.Nde

9、IdoesnotcutpET3d.pET3adcloningexpressionregionpET3adVectsTB0261298pET3a(4640bp)i(2814)Ap(35754432)ClaI(24)HindIII(29)Bpu1102I(458)BamHI(510)NdeI(550)XbaI(588)BglII(646)SgrAI(687)SphI(843)EcoNI(903)SalI(928)PshAI(993)EagI

10、(1216)NruI(1251)ApaBI(1329)BspMI(1331)BsmI(1636)AvaI(1702)MscI(1723)Bpu10I(1858)BscGI(1912)PvuII(2343)BsmBI(2393)Tth111I(2497)BsaAI(2504)Bst1107I(2523)SapI(2636)AflIII(2752)BspLU11I(2752)AlwNI(3168)HgiEII(3338)PstI(3890)

11、PvuI(4015)ScaI(4125)SspI(4449)AatII(4567)EcI(4638)ApoI(4638)Eam1105I(3645)–Cat.No.pET3aDNA694183pET3bDNA694193pET3cDNA694203pET3dDNA694213Novagen?DERING8005267319?TECHNICALSUPPT8002070144AGATCTCGATCCCGCGAAATTAATACGACTCAC

12、TATAGGGAGACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCTAGCATGACTGGTGGACAGCAAATGGGTCGCGGATCCGAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATMetAlaSerMetThrGlyGlyGlnGlnMetGlyArgGlySerGluPhe

13、LeuLysThrLysGlyProArgAspThrProIlePheIleGlyEndpET5b...GGTCGGGATCCGAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAAT...GlyArgAspProAsnSerEndpET5c...GGTCGGATCCGAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAA

14、TGTCATGATAAT...GlyArgIleArgIleLeuGluAspGluArgAlaSerEndNdeIEcIT7?TagBamHINheIpET5arbsBglIIXbaIT7promoterprimer#693483T7promoterpBR322EcIclockwiseprimeri(2308)Ap(30693926)BamHI(4)NheI(37)NdeI(44)XbaI(82)BglII(140)SgrAI(181

15、)SphI(337)EcoNI(397)SalI(422)PshAI(487)EagI(710)NruI(745)ApaBI(823)BspMI(825)BsmI(1130)StyI(1140)AvaI(1196)MscI(1217)Bpu10I(1352)BsgI(1406)BspEI(1435)PvuII(1837)BsmBI(1887)Tth111I(1991)BsaAI(1998)Bst1107I(2017)SapI(2130)

16、AflIII(2246)BspLU11I(2246)AlwNI(2662)HgiEII(2832)PstI(3384)PvuI(3509)ScaI(3619)SspI(3943)AatII(4061)EcI(4132)ApoI(4132)pET5accloningexpressionregionTB0291298ThepET5acvectscarryanNterminalT7?TagsequenceBamHIEcIcloningsite

17、s.TheT7terminatpresentonmanyotherpETvectsismissinginthepET5acseriestherefeβlactamaseisgenerallyproducedinlargeamountsinpET5recombinantsinducedfT7RNApolymeraseexpression.ThepET23ad()seriescrespondstopET5acbutincpatessever

18、aladditionalfeaturesincludingtheT7terminat(Cat.No.693373).Uniquesitesareshownonthecirclemap.NotethatthesequenceisnumberedbythepBR322conventionsotheT7expressionregionisreversedonthecircularmap.Thecloningexpressionregionof

19、thecodingstrtranscribedbyT7RNApolymeraseisshownbelow.pET5a()sequencelmarksT7promoter109125T7tranionstart108pBR322igin2308blacodingsequence30693926ThemapsfpET5bpET5carethesameaspET5a(shown)withthefollowingexceptions:pET5b

20、isa4133bpplassubtract1bpfromeachsitebeyondBamHIat4.pET5cisa4132bpplassubtract2bpfromeachsitebeyondBamHIat4.pET5acVectspET5a(4134bp)Eam1105I(3139)–Cat.No.pET5aDNA694263pET5bDNA694273pET5cDNA694283本本資資源源由由生生物物秀秀搜搜集集整整理理并并提

21、提供供下下載載,,僅僅供供學學習習參參考考☆☆☆☆☆☆☆☆生生物物秀秀[[wwwwww..bbbbiioooo..ccoomm]]☆☆☆☆☆☆☆☆中中國國生生物物科科學學論論壇壇[[wwwwww..bbbbiioooo..ccoommbbbbss]]☆☆☆☆☆☆☆☆生生物物秀秀下下載載頻頻道道[[wwwwww..bbbbiioooo..ccoommSSoofftt]]生生物物秀秀傾傾力力打打造造最最大大最最專專業(yè)業(yè)的的生生物物資資源源下

溫馨提示

  • 1. 本站所有資源如無特殊說明,都需要本地電腦安裝OFFICE2007和PDF閱讀器。圖紙軟件為CAD,CAXA,PROE,UG,SolidWorks等.壓縮文件請下載最新的WinRAR軟件解壓。
  • 2. 本站的文檔不包含任何第三方提供的附件圖紙等,如果需要附件,請聯(lián)系上傳者。文件的所有權(quán)益歸上傳用戶所有。
  • 3. 本站RAR壓縮包中若帶圖紙,網(wǎng)頁內(nèi)容里面會有圖紙預覽,若沒有圖紙預覽就沒有圖紙。
  • 4. 未經(jīng)權(quán)益所有人同意不得將文件中的內(nèi)容挪作商業(yè)或盈利用途。
  • 5. 眾賞文庫僅提供信息存儲空間,僅對用戶上傳內(nèi)容的表現(xiàn)方式做保護處理,對用戶上傳分享的文檔內(nèi)容本身不做任何修改或編輯,并不能對任何下載內(nèi)容負責。
  • 6. 下載文件中如有侵權(quán)或不適當內(nèi)容,請與我們聯(lián)系,我們立即糾正。
  • 7. 本站不保證下載資源的準確性、安全性和完整性, 同時也不承擔用戶因使用這些下載資源對自己和他人造成任何形式的傷害或損失。

評論

0/150

提交評論