氟喹諾酮復合模板印跡聚合物的制備及其在食品檢測中的應用.pdf

1、河北大學碩士學位論文氟喹諾酮復合模板印跡聚合物的制備及其在食品檢測中的應用姓名：申杰申請學位級別：碩士專業(yè)：環(huán)境科學指導教師：劉芃巖20100601Abstract IIAbstract This thesis consists of three chapters. IN CHAPTER ONE, the development history and basic principle of the molecularly imprint

2、ing technology (MIT) were described. The preparation methods of molecular imprinting polymer (MIP) and the application of MIP in food detection were also reviewed. IN CHAPTER TWO, a high-performance liquid chromatography

3、-diode array detection method (HPLC-DAD) for the determination of fluoroquinolone antibiotics in milk has been developed using molecularly imprinted polymer solid phase extraction (MISPE). The molecularly imprinted polym

4、er (MIP) has been prepared using levofloxacin and ciprofloxacin as the templates, α-methacrylic acid (MAA) as functional monomers and trimethylolpropane trimethylacrylate (TRIM) as crosslinker. The imprinted polymer has

5、been characterized by equilibrium binding experiment to investigate the binding ability to template molecule. Various parameters affecting the extraction efficiency of the polymer in the MISPE procedure have been evaluat

6、ed to achieve optimal preconcentration followed by a elution with 4% ammonia in methanol. The resides are extracted from milk with 2% acetic acid in acetonitrile, purified on the MISPE cartridge and then analyzed using H

7、PLC-DAD set at 281 nm. The method has been validated by analyzing spiked milk samples at two levels (100 and 200 μg·kg-1 ), the mean recoveries of the method range from 84.1 to 104.7% with relative standard deviatio

8、n no more than 4.3% (n=3) for fleroxacin, enoxacin, pefloxacin, norfloxacin, ciprofloxacin, levofloxacin, lomefloxacin, enrofloxacin, gatifloxacin, sparfloxacin, the method detection limits range between 1.34 and 7.35 μg

9、·kg-1. The HPLC-DAD validated method was proved to be suitable for the sensitive and accurate quantification and confirmation analysis for the residues of fluoroquinolones. IN CHAPTER THREE, a method has been establ

10、ished for simultaneous determination of residues of ten fluoroquinolones in grass carp by high performance liquid chromatography-ion trap mass spectrometry (HPLC-ITMS). The samples were extracted with 2% acetic