羅漢果蛋白酶的提取分離、特性及應(yīng)用研究.pdf

1、屋z痧吁凝靠謦碩士研究生學(xué)位論文Y78800THESlSFORMASTER’SDEGREEOFGUANGXINORMALUNIVERSI“rY羅漢果蛋白酶的提取分離、特性及應(yīng)用研究TheExtraction，Isolation，CharacteristicandApplicationofProteaseinSiraitiaGrosvenorrii院系j化學(xué)化工學(xué)院耳級影櫳級專業(yè)j有機(jī)化學(xué)研究方向天煞有機(jī)化學(xué)導(dǎo)師j蘇小建高工研究生j梁成欽

2、完成時(shí)間：2005年5月中國桂轅ABSTRACTInthispaperthedocumentsofsiraitiagrosvenoriandplantproteinasepublishedintherecentyearsinChinawereanalyzedbyapplyingthemethodsofdocumentmeterologyByevaluatingthedocumentaltypes，publishingyears，subj

3、ectfieldsandsoon，thestudiesonthesiraitiagrosvenorimadplantproteinaseweresummarizedandthecurrentstatusanddevelopingdirectionforlaterresearchwerealsopointedoutAnewplantenzymeinsiraitiagrosvenoriiwasfirstdiscoveriedandstudi

4、edinthispaperDuringtheexperiments，thestudiesontheconditionsofextractingenzyme，thecharactersofpurifiedenzymeandenzymekineticparameters，andapplicationofhydrolyzingsoybcanisolatedproteinsweremainlyconcernedaboutThroughthese

5、methods，wegotthedataoftheenzyme’scharactersandmuchusefulinformationaboutapplicationTheseresultscanprovidetheoreticalfoundationforfurtherstudyandindustrialuseofthiskindofenzymeDuringthestudiesonextractionandisolation，thre

6、ekindsofmethodofextractingbyalcohol，tannin，andultrafilration(UF)wereintroducedandcompariedAndtheproteasewaspurifiedbyDEAESepharoseCL6BchromatographyAtthesametime，thepropertiesofthepurifiedproteasewerestudiedTheresultssho

7、wedthattheenzyemobtainedbyultrafiltrationhasthebestqualityandhighactivityAndasinglebandwasfoundinthegelofisoelectricfocusingwiththepurifiedproteaseItsspecificactivityWas34759x104U／g，itspurificationploidyreached187foldcom