簡介：復旦大學碩士學位論文黃瓜鈍綏螨AMBLYSEIUSCUCUMERIS生物學與生態(tài)學若干問題的研究姓名李新義申請學位級別碩士專業(yè)動物學指導教師楊琰云吳千紅200051●堡竺塑蘭竺竺皂苧查竺蘭竺壁蘭奠蘭BIOLOGICALSTUDIESINCLUDINGLABORATORYPOPULATIONLIFEPARAMETERSOFTHEPREDATORYMITEAMBLYSEIUSCUCUMERISACARINAPHYTOSEIIDAEABSTRACTTHEVARIOUSBIOLOGICALASPECTSANDLIFEHISTORYPARAMETERSWERESTUDIEDSYSTEMATICALLYBYPRESENTPAPER1THEBIOLOGICALTRAITSINTHELIFESPANWASOBSERVEDANDDESCRIBED2THEEFFECTOFDEPRIVATIONFOODOFACUCUMERISONTHEPREDATORYCAPABILITYWASSTUDIEDTHETIMEOFDEPRIVATIONOFFOODHASDEFINITELYEFFECTONTHEPREDATIONBEHAVIORACERTAINTIMEDERIVATIONOFFOODHASEF琵CTINACERTAINPERIODTHEPREDATORYCAPABILITYOFMATEDADULTFEMALEACUCUMERISWASDEVELOPEDWITHTHEAGEINCREASINGTILLREACHTHEMOSTSTRONGEST3THESERIESFUNCTIONATRESPONSESOFVARIOUSSTAGESOFACUCUMENSONTHEEGGSOF7KJROPHAGUSPUTRESCENTIAESCHRANKANDONTHEADULTFEMALEOFPOLYPHAGOTARSONEMUSLATUSBANKWERESTUDIED，ANDTHEHOILINGDISCEQUATIONWASAPPLIEDA11THEFUNOTIONALRESPONSESAREBELONGTOTYPEIIAMONGTHEPRTONYMPH，DEUTONYMPHANDMATEDADULTFEMALE。THEADULTMATEDFEMALEISTHEMOSTEFFICIENTPREDATORYSTAGE，ANDTHEATTACKRATE嘲ISTHEHIGHESTANDTHEHANDLINGTIME錨ISTHESHORTESTONBOTHEGGSOFZPUTRESCENFFAEANDMATEDADULTFEMALEP1ATUSTHEDEUTONYMPHRANKSTHESECONDEFFICIENTPREDATORYSTAGETHEEFFICIENCYOFPREDATIONOFACLLCTLMER／SONTHEPATUSMAKESTHESPECIESAPOTENTBIOLOGICALCONTROLAGENTTOCONTROLTHEPATUSINGREENHOUSE4THEDEVELOPMENTALTHRESHOLDTEMPERATUREANDACCLIMATIZATIONWEREPRESENTEDACCORDINGTOTHEDEVELOPMENTTIMEATVARIOUSTEMPERATURESTHEDEVELOPMENTALTIMEISDECREASINGASTHETEMPERATUREINCREASING，ANDHASTHEHYPERBOLICRELATIONWITHTHEVARIETYOFTEMPERATURE5FEDONZPUTRESCENTIAEATVARIOUSTEMPERATURESNETREDRODUCTIONRATER，INTFINSICRATEOFNATURALINCREASERM，F(xiàn)INITERATEOFINCREASE，DAYSFORPOPULATIONTODOUBLET，MEANGENERATIONTIME丁，LONGEVITY，TOTALFECUNDITY，F(xiàn)ECUNDITYRATEANDEGGEGGDEVELOPMENTALTIMEOFTHEPOPULATIONOFAC擬CLCMERISA11HAVEAPARABOLARELATIONWITHTEMPERATUREWHILETHETIMETO50％MORTALITYANDDEVELOPMENTALTIMEHAVEHYPERBOLICRELATIONZPUTRESCENTIAEISMORESUITABLETHENSPIDERMITEASFOODTOPROLIFERATEACUCULYLERIS25℃ISTHEMOSTFAVORABLEAMONGA11TEMPERATURES，RMIS02051WHICHISHIGHERTHANPAPERSANDTHESALTIEPARAMETERPRESENTEDBYSPIDERMITEASFOODKEYWORDSBIOLOGICALCONTROLBIOCONTR01，AR曲LYSEIUSCⅡC“，NES，F(xiàn)UNCTIONALRESPONSES，LIFETABLE2

簡介：東北農(nóng)業(yè)大學博士學位論文豬囊尾蚴的發(fā)育生物學及藥物作用機理姓名劉永杰申請學位級別博士專業(yè)基礎獸醫(yī)學指導教師李慶章200051劉水杰英文摘要2000年5月DEVELOPMENTALBIOLOGYOFCYSTICERCUSCELLULOSAEAND枷CHANISMOFACTIONOFDRUGSABSTRACTTSOLIUMCYSTICERCOSISISANIMPORTANTPARASITICZOONOSISENDANGERINGTHEDEVELOPMENTOFHUSBANDRYANDHEALTHYOFHUMANINORDERTOSELECTANEFFECTIVEMEDICINEAGAINSTCYSTICERCOSISANDTOPROVIDEATHEORETICABASISFORCHEMOTHERAPY，THEAUTHORSTUDIEDFIRSTLYANDSYSTEMATICALLYDEVELOPMENTOFCYSTLCERCIINVIVOANDINVITROANDTHEEFFECTOFDRUGSONCYSTICERCIINDLFFERENTDEVELOPMENTALSTAGESBYOPTICAMICROSCOPEANDELECTRONICMICROSCOPETHEMORPHOLOGYANDSTRUCTUREOFCYSTIEERCIWEREOBSERVEDINDETAILHTECHNIQUE，INSITUEND一1ABELLING，WASAPPLIEDTODETECTQUALITATIVELYANDQUANTITATIVELYTHEAPOPTOTLCEELLPOSITIVEINDEXAIINCYSTICERCIANDHOSTSINCOMBINATIONWITHTHEDETECTIONOFTHE1EVELSOFNITRICOXIDENOANDAIOFLYMPHOCYTELAI，THECLINALGENESISOFCYSTICERCOSISANDPOSSIBLEMECHANISMOFACTIONOFTHEDRUGSAGAINSTCYSTICERCIWEREANALYZEDTHERESULTSWERESHOWEDASF0110WS1ANINVITROCULTURESYSTEM1NCORPORATINGACELLMONOLAYERWITHOUTGASBHASEWASFIRSTLYUSEDTOINDUCEMOSTOFTHEONCOSPHERESOFTSOLIUMTOENTERDOSTONCOSPERALDEVELOPMENTTHE1ARVALCANSURVIVEFOR16DAYS2INVITROHATCHEDONCOSPHERESWEREINJECTEDINTRAVENOUSLYINTOTHEMICETHEMJCEWEREAUTOPSIED2MONTHSAFTERINOCUFATIONCYSTICERCIWEREFOUNDINTHE1UNGAND56PERCENTMICEWEREINFECTEDTHEMICEINFECTEDINTRAVENOUSLYBYONCOSPHERESAREVALUABLEEXPERIMENTALANIMAMODELSFORSTUDYINGTS01IUMCYSTLCERCOSIS3CHANGESOFMORPH0109Y，HIST0109ICALSTRUCTUREANDULTRASTRUCTUREOFCCEL1ULOSAEINTHEDEVELOPMENTWEREFIRSTIYANDSYSTEMATICALLYSTUDIEDTHESIZEOFCYSTICERCIINTHESAMEHOSTWEREVARIABETHEYOUNGESTCYSTICERCIOBSERVEDWEREI9DAYS01D，BUTSUCKERSANDHOOKSWERENOTFOUNDINSC01EXREGIONHISTOLOGYSHOWEDTHATALOTOFCELLSACCUMULATEDATONEOFTHEPOLESANDATTHEPROLLFERATIONREGIONTHETEGUMENTINVAGINATEDWITHTHEDURATIONOFINFECTION，SCOLEXBEGANTODIFFERENTIATEANDPOSSESSEDSUCKERSANDHOOKSDURINGTHEDEVELOPMENTTHEBLADDERWALLISATRANSITORYEMBRYOONTHEBASISOFWHICHTHESC01EXDEVELOPS60DAYS】ATER，MOSTOFCYSTICERCIWEREMATURETHEULTRASTRUCTURESHOWEDTHECOMPONENTSOFCYSTICERCIINCREASEDINNUMBERANDEXTENTBUTNONEWCELLSORTISSSUESAPPEAREDASTHECYSTICERCIGREWBLADDERWALLANDSOOLEXHADSOMEDIFFERENCESINULTRASTRUCTURESTHEBLADDERWALLSEEMSTOHAVEDIFFERENTFUNCTIONSFROMSCOLEXTHEBLADDERWALLMIGHTFUNCTIONINTHEABSORPTIONOFNUTRITIONWHILETHESCOLEXWASCONCERNEDWITHTHEFUTUREDEVELOPMENT2

簡介：近年來減毒細菌活載體因其能夠刺激機體產(chǎn)生針對活載體及其攜帶的外源抗原的體液、細胞免疫和局部粘膜免疫受到人們的廣泛關注特別是近期研究結果證明許多活細菌載體能夠作為DNA疫苗的運送載體可以通過口服減毒細胞進入機體從而誘導產(chǎn)生針對特定抗原的各種免疫應答但迄今為止對活疫苗載體與宿主免疫系統(tǒng)間相互作用規(guī)律及其調(diào)控機制知之甚少這方面的免疫生物學研究對于研制新型基因工程疫苗將會提供重要的理論依據(jù)且對相關疾病的免疫防制有著重要的指導價值因此我們構建了表達綠色熒光蛋白GFP的減毒鼠傷寒沙門氏菌闡明其在體內(nèi)、外感染試驗的動態(tài)分布及它與抗原呈遞細胞的相互作用規(guī)律這為沙門氏菌疫苗載體及相關基因工程疫苗的研究奠定了理論基礎1表達綠色熒光蛋白的重組減毒鼠傷寒沙門氏菌的構建與鑒定2重組減毒鼠傷寒沙門氏菌感染動力學3重組減毒鼠傷寒沙門氏菌與抗原呈遞細胞的相互作用綜上所述該研究構建了表達綠色熒光蛋白的鼠傷寒沙門氏菌通過靜脈注射和口服途徑記錄了此細胞在體內(nèi)一些臟器的分布規(guī)律及誘導的抗體的變化結果顯示細菌數(shù)量及抗體效價變化均有一定的規(guī)律而且細菌數(shù)量與抗體效價之間有一定的相關性在體外用細胞系和腹腔巨噬細胞驗證了此細菌具有感染細胞的能力在體內(nèi)感染實驗中檢測了靜脈注射途徑肝臟、脾臟中巨噬細胞和樹突細胞的感染率同時檢測了口服后3天腹腔巨噬細胞的感染狀況結果顯示在體內(nèi)巨噬細胞和樹突細胞很快地攝取了該重組菌這為起始保護性免疫反應奠定了基礎

簡介：山東農(nóng)業(yè)大學博士后士學位論文蘋果角質(zhì)層的生物學和虎皮病的發(fā)生姓名張元湖申請學位級別博士后士專業(yè)果樹學指導教師束懷瑞2001121內(nèi)部結構已經(jīng)被破壞。在表皮細胞及下皮細胞周圍也有有大量電子密致物，電子密致物進入細胞時伴有小囊泡的形成，細胞內(nèi)部結構包括質(zhì)體被破壞。貯藏至20周時，晚采果仍然沒有發(fā)生虎皮病，果皮細胞結構完整，仍然可以見到質(zhì)體及線粒體。超微結構中僅見表皮細胞上方有大量電子密致物，而表皮細胞下方只有少量電子密致物，且未或僅小部分接觸細胞。外層下皮細胞周圍未見有下移電子密致物，細胞內(nèi)部結構完好，葉綠體結構完整。總之，角質(zhì)層中的電子密致物在蘋果虎皮病發(fā)生過程中可向內(nèi)層表皮細胞、下皮細胞移動，從外部破壞細胞，使細胞解體，產(chǎn)生褐變。推測角質(zhì)層中蠟層的消失及角質(zhì)層電子密致物的下移與小法尼烯的積累和氧化有關。研究表明在貯存過程中早采和晚采國光和紅星蘋果角質(zhì)層中的共軛三烯含量與虎皮病的發(fā)生存在不相關現(xiàn)象，進一步證明了以共軛三烯含量來表示僅法尼烯氧化產(chǎn)物說明虎皮病的發(fā)生程度存在弊端。而RFC值與虎皮病的發(fā)生密切相關。RFC值可以表示角質(zhì)層中抗氧化水平。晚采和DPA處理的果實的抗氧化活性分別顯著高于早采果和未處理果，前者的RFC值也分別顯著高于后者。修正了A一法尼烯與共軛三烯的比值RFC值用于虎皮病預測的數(shù)學模型。模型可用于貯存過程中蘋集虎皮病的預測。通常在RFC值低于10時蘋果出庫至常溫時虎皮病即會發(fā)生弋關鍵詞蘋果；角質(zhì)層；蠟層；超微結構；虎皮病；電子密致物；旺法尼烯；共軛三烯II

簡介：中國科學技術大學碩士學位論文同步輻射小麥引起的生物學效應血源與基因工程Α干擾素抑制貯脂細胞增殖及膠原合成的比較研究姓名趙凌申請學位級別碩士專業(yè)細胞生物學指導教師顧月華200141中國科學技術人學預上學位論文第一部分同步輻射對小麥引起的生物學效應摘要研究了不同劑量的同步輻射軟X射線源輻照小麥種子后產(chǎn)生的生物學效應，以期從細胞和分子水平蛋白質(zhì)、酶及DNA等上揭示同步輻射軟X射線源對生物體的誘變效應及其作用機制，為其應用于輻射育種提供理論依據(jù)。I我們進行了以下研究I檢測小麥幼苗細胞的脂質(zhì)過氧化作用及保護酶活性?？梢钥闯?，隨著同步輻射軟X射線劑量的增加，脂質(zhì)過氧化產(chǎn)物丙二醛MDA的含量也隨之增加；在保護酶系統(tǒng)中，過氧化氫酶CAT、過氧化物酶POD、超氧化物歧化酶SOD的活性變化不表現(xiàn)出同步性，表明三者之間存在相互協(xié)調(diào)的關系，處理組葉綠素含量及葉綠體中關鍵的保護酶抗壞血酸過氧化物酶APX的活性始終低于空白對照組。結果表明同步輻射軟X射線輻照對于小麥細胞保護酶活性及脂質(zhì)過氧化作用的影響很大。II采用單細胞凝膠電泳方法檢測了同步輻射軟X射線源對DNA造成的單鏈損傷，通過測定DNA遷移效應，表明DNA損傷程度與輻照劑量呈顯著的正相關性。1ID采用TUNEL方法來進行原位細胞凋亡檢測，并結合DAPI熒光染色，表明同步輻射軟X射線源輻照小麥種子可以使之產(chǎn)生不同程度上的細胞凋亡。通過統(tǒng)計細胞凋亡率，也表明損傷程度與輻照劑量成『F相關性。以上實驗表明，作為一種新的誘變源，同步輻射軟X射線輻照麥類作物種子的生物學效應顯著，它不僅能夠?qū)π←溂毎z傳物質(zhì)造成損傷同時也能對相關的體內(nèi)保護酶系統(tǒng)產(chǎn)生調(diào)節(jié)作用，因此應用同步輻射進行農(nóng)業(yè)育種將有、極大的發(fā)展?jié)摿颓熬啊?關鍵詞同步輻射軟X射線源；小麥細胞；脂質(zhì)過氧化；保護酶系統(tǒng)；單細胞凝膠電泳；細胞凋亡

簡介：華中農(nóng)業(yè)大學碩士學位論文湖北省玉米粗縮病病原分子生物學鑒定及玉米抗感病性研究姓名燕永亮申請學位級別碩士專業(yè)植物病理學指導教師侯明生于嘉林200251ABSTRACTMAIZEISONEOFTHEMAINCROPSINCHINA，ANDISALSOTHEIMPORTANTSUSCEPFIDEMATERIALFORFORAGE，LIGHTINDUSTRYANDMEDICINENOTONLYTHETOTALPLANTATIONAREABUTALSOTHEYIELDAREALLINTHETHIRDPLACE，ONLYBELOWTHATOFRICEANDWHEATINRECENTYEARS，WITHTHECHANGEOFTHECULTIVATEHABBITTHEPLANTATIONARCABECOMEBIGERANDBIGERANDTHESPECIESCHANGINGFIEQUENTLYOWINGTOTHEFARMERSIMPLYSEEKHIGHYIELDANDPLANTSENSITIVEMAIZESPEIEES，THUSCAUSEASERIESOFDISEASESBECOMEHIGHERTHANEVERWHILE，THEDISEASESNEVERHAPPENEORSELDOMHAPPENBEFOREONEOFTHESEEXAMPLESISTHEMAIZEROUGHDWARFDISEASEIT’SREPORTEDTHATTHEPATHOGENYCAUSEDTHEDISEASEISMRDVANDWHICHBELONGSTOTHEREOVIRIDAEFIJIVIRUSTHEVIRUSISSOSIMILAR誦也ANOTHER丘JIVIRUSRBSDVINBIOLOGIEALANDIMMANITICALCHARACTERTHATMANYSPECIALISTSREGARDTHESETWOVIRUSESASONETHROUGHANALYZINGTHENUCLEOTIDESSEQUENCEOFTHESEVIRUSES，WECANCLASSFYTHEMCORRECTLYONEISOLATEOFTHEPATHOGENYFROMHUBEIWASANALYZEDATTHEMOLECULARLEVELTHEGENOMICRNAELEETROPHORETICPROFILESSHOWEDTHEGENOMECOMPOSED10DSRNASEGMENTSSELECTTHES7，CLONETHEEDNABYRTPCRAMPLICATION，ANDTHENSEQUENCETHECLONEWHICHHASREGISTEDINTHEGENBANKANDTHENUMBERAF397874THERESULTOFTHESEQUENCEANALYSISDEMONSTRATEDTHATTHEGANOMEOFHUBEIISOLATEHASTHEHOMOLOGY、訪TLLMRDVIANDRBSDVJFOR853％AND937％ALSOTHETWOORFSINTHISSEQUENCEHASTHEHOMOLOGYWITHTHATOFMRDVIANDRBSDVJFORORFL，908％AND98％；ORF2，854％AND957％THERESNLTINDICATEDTHATTHEVIRUSWHICHCAUSETHISMAIZEDISEASEINHUBEIISRICEBLACKSTREAKEDROUGHVIRUSBASINGONTHESTUDYATMOLECULARLEVEL，WEALSOIDENTIFIEDTHERESISTENCEANDSENSITIVETOTHEDISEASEWITH64MAIZESPECIESINFIELDINTIANMENANDHANCHUANWHERETHEDISEASEWASVERYHEAVYFORSEVERALYEARSTHESEMAIZESPECIESWERECOLLECTEDFORMLIAONINGBEIJING，SICHUANANDSHANDONGPROVINCESTHERESULTSHOWSTHATTHEREARE21SPEIEESHAVE1LIGHRESISTENEETOTHEDISEASE，2SPEICESHAVEHIGHSENSITIVETOTHEDISEASE，THEOTHERSAREINTHEMIDDLEINASENSE，THERESULTISVERYIMPORTANTBECAUSEITMAYHELPTHEFARMERTOSELECTTHESUITABLESEEDKEYWORDSRICEBLACKSTREAKEDDWARFVIRUSMAIZEROUGHDWARFDISEASEEDNACLONINGSEQUENCEANALYSIS2

簡介：福建農(nóng)林大學碩士學位論文EUI基因?qū)﹄s交稻若干生物學特性和構成產(chǎn)量因素的影響姓名章清杞申請學位級別碩士專業(yè)作物遺傳育種指導教師楊仁崔200251關鍵詞EUIL基因；EUI2基因；E．雜交稻；農(nóng)藝性狀；產(chǎn)量構成；干物質(zhì)積累配合力

簡介：四川農(nóng)業(yè)大學碩士學位論文老鷹茶白粉菌生物學特性、侵染過程及抗性變化的初步研究姓名朱蓮申請學位級別碩士專業(yè)森林保護指導教師劉應高20070601ABSTRACTPOWDERYMILDEWISADETRIMENTALDISEASEOFEAGLETEALITSEACOREANALEVLVARLANUGINOSEINTHECOMMERCIALGROWINGAREAINYAAN，SICHUANPROVINCETHISDISEASEINDUCESABNORMALLEAFSHAPESANDADVERSELYAFFECTSTHEEAGLETEAPRODUCTIVITYTHESTUDYRECORDEDANDDEMONSTRATEDTHESYMPTOMSANDSIGNSOFTHISDISEASEINDETAILS，ANDSYSTNNATICALLYSTUDIEDTHEPATHOGENICFIMGUS’SBIOLOGICALCHARACTERISTICSPATHOGENHOSTINTERACTIONS，ANDHOSTPLANTPHYSIOLOGICALANDBIOCHEMICALDEFENSERESPONSESACCORDINGTOTHEMORPHOLOGICALCHARACTERISTICSINCLUDINGTHESIZESOFTHECONIDIOPHOREANDCONDIUM，THEPATHOGENWASIDENTIFIEDASOIDIUMOIDIUMCALLGROWBOTHINTHEACIDORALKALINEENVIRONMENTTHESPOREGERMINATIONCANHAPPENEDINPHRANGES30120，ANDWITHTHEOPTIMUMPHFORSPORULATIONAT100THEOPTIMUMTEMPERATUREFORSPORULATIONRANGED15TO25“C，ANDTHEOPTIMUMHUMIDITYIS100％DARKORINDIRECTILLUMINATIONSAREADVANTAGEOUSTOSPOREFORMATIONTHEULTRAVIOLETLIGHTUVLISDEVASTATINGTOOIDIUMSPORE，ANDTHELONGERTHEUVLILLUMINATIONTHELESSTHEGERMINATIONTHISSTUDYSHOWEDTHATTHEREWERENOSPOREGERMINATIONAFTERTREATEDWITHUVLFOR30MININTHESTUDY。RESISTANCETOPOWDERYMILDEWCAUSEDBYPATHOGENICFUNGUSOIDIUMOF5COMMERCIALLYIMPORTANTEAGLETEACULTIVARSINSICHUANPROCINCEWEREEVALUMEDBOTHINTHEFIELDANDINTHELABORATORYONARTIFICIALLYINOCULATEDPLANTSRESULTSWERECONSISTENTFORTHETWOEVALUATIONMETHODS，ANDTHEREFORE，WECONCLUDEDTHATARTIFICIALLYINOCULATIONINTHELABORATORYISRELIABLEANDFEASIBLE，ESPECIALLYWHENALARGEAMOUNTOFCULTIVARSWEREINVOLVEDOUREVALUATIONSFOUNDTHAT，AMONGFIVEEAGLETEACULTIVARS，ONECULTIVARWASHIGHLYRESISTANT，TWOCULTIVARSWERESUSCEPTIBLE，ANDTHERESTTWOCULTIVARSWEREMEDIUMRESISTANTINTHESTUDY，ELECTRONICSCANNINGMICROSCOPEANDOPTICALMICROSCOPEWEREUSDTOOBSERVETHEHOSTPATHOGENHISTOLOGICALINTERACTIONSINDIFFERENTPHASESTHERESULTSSHOWEDTHATAT4HAFTERINOCULATION，THEPRIMARYGERMTUBESWEREEMERGEDFROMTHEGERMINATINGSPORESAT8HAFTERINOCULATIONTHEAPPRESSORIUMGERMTUBEFORMEDAPPRESSORIUMATL2HAFTERINOCULATIONAPPRESSORIUMFORMEDAPPRESSORIUMLOBEORSECONDAPPRESSORIUMLOBEANDAPPRESSOFIUMINDUCEHOSTCELLSFORMEDHALOAT24HAFERINOCULATIONAPPRESSORIUMDEVELOPEDFURTHERINTOHAUSTORIAAT48HAFTERINOCULATION，THEPRIMARYHYPHAEGREWFROMTHEAPEXOFTHEAPPRESSORIUMOVERTHESURFACEOFTHEHOSTEPIDERMALCELLSAT72HAFTERINOCULATIONPRIMARYHYPHAEDEVELOPEDINTOSECONDARYHYPHAETHETIMECONRSECHANGESINTHEACTIVITYOFPHOTOSYNTHESIS，PEROXIDASEPOD，CATALASELI

簡介：中國農(nóng)業(yè)大學碩士學位論文毒害艾美耳球蟲晚熟系選育及其生物學特性研究初報姓名董輝申請學位級別碩士專業(yè)預防獸醫(yī)學指導教師索勛汪明200261中嗣農(nóng)業(yè)太學頸士學位論文EIMERIANECATRIXSELECTIONANDCHARACTERISTICSOFAPOSTCOCIOUSLINEABSTRACTASTRAINOFENECATRIXWASISOLATEDFROMSUSPENSIONSOFOOCYSTSBYTHEUSEOFTHESINGLEOOEYSTISOLATIONTECHNIQUETHEOOCYSTSOFTHESTRAINWEREELLIPTICA／INSHAPEANDHADAVERAGEDIMENSIONSOF19。50圭2。31X15。8L圭186NMITSPREPATENTPERIODWAS138HANDTHEPEAKOFOOEYSTPRODUCTIONOECUREDONTHE7THPOSTINFECTIONSTUDYOFITSPATHOGENECITYSHOWEDTHATTHETIMEANDDEGREEOFCLINICALSIGNSWEREDIFFERENTANDESS∞IATADWITHTHEDOSESINOCULATEDANDALLDOSESHADNEGATIVEEFFECTONTHEWEIGHTGAI娜OFTHEBIRDSANDCAUSEDDIFFERENTINTESTINALLESIONS一APOSTCOCIOUSLINEOFTHESTALINWASSELEETADBYREPEATEDPASSAGESOFOOCYSTSTHATWERELATELYCOLLECTEDFROMFAECESOFPREVIOUSLYINFECTEDCHICKENSCOMPAREDWITHTHEPARENTSTRAINTHEPOSTCOEIOUSLINEHADTHEFOLLOWINGCHARACTERISTICSTHEPEAKPRODUCTIONOFOOCYSTSWASTWODAYSLATER；THEPREPATENTPERK堪WASNOTPROLONGED，BUTTHEDIMENSIONOFSEVERALENDOGENOUSDEVELOPMENTSTAGESWASLARGERTHANTHATOFTHEEORRESPOND趣GS紐GESOFTHEPARENTSTRAIN；THEPATHOGENICITY鋪磕SSIMILARINCOMPARISONWITHTHEPARENTSTMIN；THEREPRODUCTIVECAPACITYANDTHEIMRAUNOGENICITY蝴HTGHRTHANAAATOFTLWPARENTSTRAINRESPECTIVELYKEYWORDSE矗NERTANECATRIX；POSTEOEIOUSLINE；SELECTION；CHARACTERISTICS2

簡介：中國農(nóng)業(yè)大學碩士學位論文綿羊胚胎期血漿促性腺激素生物學活性的變化和垂體細胞體外無血清培養(yǎng)模型的建立姓名曹興元申請學位級別碩士專業(yè)生理學指導教師崔勝200261里奎些查堂墮主絲苧90、120天和分娩時分別為10．190．27NG／ML、9．320．13NG／ML、7．320．21NG／ML、7．02／0．29N咖1?！危蠜把芯拷Y果提示『血漿B．FSH和B．LH在妊娠前期生物學濃度較骶，在妊娠中期生物學濃度達到最高，隨后生物學活性下降或保持恒定。并且雌雄胚胎BFSH和BLH之問存在著性別差異。與先前RIA方法對FSH和LH的研究相比，存在著較大的差異，說明促性腺激素生物學活性的測定方法比RJA方法更能準確反應胚胎期FSH和LH的生理功能。此外，本實驗還建立了綿羊垂體細胞體外無血清培養(yǎng)模型，并且對培養(yǎng)不同時間的垂體細胞進行了LHB的免疫細胞化學染色，以檢測培養(yǎng)過程中垂體細胞自主分泌LH的能力。結果在無血清培養(yǎng)4天后的垂體細胞，LHFL免疫陽性染色的細胞顯著性增加，說明此時垂體細胞具有較強的LH合成能力?！螇騊切紹論『2關鍵詞卵泡刺激素，促黃體生成素，綿羊胚胎，生物學活性，細胞培養(yǎng)，垂體，”1。。VX

簡介：浙江大學博士學位論文CPPU誘導瓠瓜單性結實生理和分子生物學機制研究姓名李英申請學位級別博士專業(yè)蔬菜學指導教師喻景權朱祝軍200241株光合作用增強，且CPPU處理株光合作用的增加更為顯著。這可能與CPPU處理后庫強的增強有關。4未授粉果和授粉果中的IAA和TZEATIN濃度在花后9D內(nèi)均呈上升趨勢，而在CPPU誘導的單性結實果中IAA濃度則呈明顯的下降趨勢，其濃度明顯低于未授粉果和授粉果實中的濃度，TZEATIN濃度則在花后3天達到最大值隨后快速下降。5NAA和GA3處理未經(jīng)授粉的瓠瓜子房不能顯著促進其細胞分裂和膨大而不能產(chǎn)生單性結實果，而CPPU處理未經(jīng)授粉的瓠瓜子房則使其細胞數(shù)目和大小以及單果重都顯著高于未授粉和人工授粉果。6測定了瓠瓜果實組織中的兩條D一型周期蛋白同源基因的CDNA全長序列。經(jīng)序列的同源性及系統(tǒng)發(fā)生分析比較，它們均屬D3周期蛋白基因，且分別命名為LAGLE；CYCD3；1和LAGLE；CYCD3；2。其推測的氨基酸序列都含有一個RETINOBLASTOMABINDINGMOTIF和PESTDESTRUCTIONMOTIF。授粉受精后，LAGLE；CYCD3；12轉錄水平增加。CPPU處理也使其轉錄水平增加且作用效果更為迅速顯著。7測定了瓠瓜果實組織中的一條酸性轉化酶基因的CDNA全長序列，長2164BP，含1992BP的編碼框，編碼663個氨基酸。經(jīng)序列的同源性及系統(tǒng)發(fā)生分析比較，該酸性轉化酶基因?qū)儆谝号菪退嵝赞D化酶，并命名為LIAL。CPPU處理或授粉受精都促進了酸性轉化酶的轉錄水平和酶活性。關鍵詞CPPU，瓠瓜，單性結實，座果率，果實發(fā)育，細胞分裂，內(nèi)源激素，源庫關系，光合作用，14C小乜物，C02加富，酸性轉化酶，C≯CD3

簡介：大連水產(chǎn)學院碩士學位論文海灣扇貝自交系生物學的初步研究姓名劉述錫申請學位級別碩士專業(yè)水產(chǎn)養(yǎng)殖指導教師張國范200261劉述錫碩士論文海灣扇貝自交系生物學的初步研究作用。5BSDL系列親本的殼長都分別高于其對應自交子一代成貝的殼長均值，除了BSDLL4外，親本的鮮重都分別高于其對應自交子一代成貝的鮮重均值。BSDL系列自交系成貝的殼長的標準差在322928范圍內(nèi)變化，鮮重標準差在235880范圍內(nèi)變化，較高的方差變化給定向選擇提供了條件。6BSDL系列自交系成貝越冬成活率最高為143％，最低為0，平均越冬成活率為53％，而對照系的越冬成活率僅為295％。表現(xiàn)了自交系在低溫海域的越冬優(yōu)勢。7BSDL07養(yǎng)成后發(fā)現(xiàn)其顏色性狀出現(xiàn)分離，成活的成貝共61個，其中20個紅褐色，41個黑色，比例近似為21。顏色性狀是一個較易觀察的性狀，如能穩(wěn)定的遺傳下去，可以建立不同的品系，本實驗為今后的海灣扇貝的顏色品系的建立提供了一個鋪墊。、J／。’關鍵詞海灣扇貝7不同遺傳毒甄，自交系浮游幼蟲_生長，養(yǎng)成生長，越冬成活率

簡介：福建農(nóng)林大學碩士學位論文腐食酪螨TYROPHAGUSPUTRESCENTIAESCHRANK生物學及抗藥性機理研究姓名于曉申請學位級別碩士專業(yè)農(nóng)藥學指導教師范青海200251福建農(nóng)林大學碩士學位論文于曉腐食酪螨生物學及抗藥性杌理研究來特靈和樂斯本，其LC50值分別為3204793MGLL、1269697MGL。1090436MGL～。Ⅱ一和5磷化氫對腐食酪螨敏感種群的熏蒸毒力為284MIRI，室內(nèi)抗性選育至16代，抗性增加到475倍?？剐苑N群的LT50與選育代數(shù)的回歸曲線方程為Y35970211120XRO9881。6不同種群體內(nèi)蛋白質(zhì)含量差異顯著，并且蛋白質(zhì)含量隨抗性水平的提高而逐漸增加，抗性種群蛋白質(zhì)含量與其抗性倍數(shù)的回歸曲線方程為YO395300909XRO9515。7腐食酪螨抗磷化氫機理之一是體內(nèi)羧酸酯酶和乙酰膽堿酯酶的活性發(fā)生變化。羧酸酯酶和乙酰膽堿酯酶的活性隨抗性水平的提高而增強?？剐苑N群羧酸酯酶比活力、乙酰膽堿酯酶比活力與抗性倍數(shù)的回歸曲線方程分別為Y7152676757XR209515、YO039900635XR09714。文／關鍵詞；腐食酪螨I有效積溫；毒，歡敏感種群；抗性種群I抗性機理

簡介：浙江大學博士學位論文BT水稻對靶標害蟲二化螟的生物學與病理學效應姓名王世貴申請學位級別博士專業(yè)農(nóng)業(yè)昆蟲與害蟲防治指導教師胡萃200251外．隨取食時間延長，各類血細胞數(shù)量DHC及血細胞總數(shù)THC均呈遞減的趨勢。從各類血細胞所占血細胞總數(shù)的百分比來看，漿血胞在取食后比例下降，而顆粒血胞則比例增加，其余珠血胞、囊血胞的變化不明顯。5．BT水稻對二化螟腸道消化酶的影響。結果表明，二化螟幼蟲腸道消化液包括兩大類蛋白酶，即類胰蛋白酶和類胰凝乳蛋白酶。幼蟲取食BT水稻后弱堿性類胰蛋白酶和類胰凝乳蛋白酶的活性總體呈下降趨勢，而強堿性類胰蛋白酶在攝入BT水稻后活性則呈上升趨勢且高于對照組。6．BT水稻對二化螟幼蟲取食行為的影響。結果表明，幼蟲對對照的選擇系數(shù)顯著高于、甚至極顯著地高于BT水稻。而在兩種BT水稻品系間，幼蟲對克螟稻1號的選擇系數(shù)要高于克螟稻2號，與它們各自的抗蟲性水平是相一致的。二化螟3齡幼蟲在轉BT基因水稻上的鉆蛀次數(shù)、蛀入時間等方面都高于對照，而蛀入成功率則比對照為低，但差異都不顯著。在鉆蛀部位的選擇上，幼蟲都表現(xiàn)出喜好植株莖桿部位，其次是葉片主脈。關鍵詞BT水稻，二化螟，生長發(fā)育，致死效應，亞致死效應，組織病理，免疫系統(tǒng)，取食行為III

簡介：華中農(nóng)業(yè)大學碩士學位論文珍珠貝的精子生物學及其在轉基因技術中的應用姓名蔡云川申請學位級別碩士專業(yè)水產(chǎn)養(yǎng)殖指導教師龔世園江世貴200251SPERMATOZOABIOLOGYOFPEAITLOYSTERSPTERIIDAEANDITSAPPLICATL0NT0TRANSGENLCTECHNOLOGYABSTRACTTHEPARENTSOFPINCTADAMARGARITIFERA，PETRIAPENGUINANDPINCTADACATAWEREDISSECTEDFORSPERMATOZOACOLLECTION，ANDTHEEXPERIMENTSOFACTIVATINGSPERMATOZOAWERECONDUCEDUNDERDIFFERENTPH，SALINITYANDWATERTEMPERATUREFRW．THERESULTSSHOWEDTHEACTIVATINGCONDITIONSFORTHESPERMATOZOAFROMDISSECTEDPINCTADAMARGARITIFERAWEREBELOWSUITABLEPHWAS9．510．0，THEOPTIMUMPHWAS9．5SUITABLESALINITYWAS2535；SUITABLESEAWATERTEMPERATUREWAS2530℃．THEACTIVATINGCONDITIONSFORTHESPERMATOZOAFROMDISSECTEDPETRIAPENGUINPARENTSWEREBELOWSUITABLEPHWAS9．09．5；SUITABLESALINITYWAS2535，THEOPTIMUMSALINITYWAS30；SUITABLETEMPERATUREWAS2530。C．THEACTIVATINGCONDITIONSFORTHESPERMATOZOAFROMDISSECTEDPINCTADAFUCATAPARENTSWEREBELOWSUITABLEPHWAS9．09．5，THEOPTIMUMPHWAS9．OSUITABLESALINITYWAS2535，THEOPTIMUMSALINITYWAS3035；SUITABLETEMPERATUREWAS2530。C，THEOPTIMUMTEMPERATUREWAS25。C．THEARTIFICIALINSEMINATIONCONDITIONOFPINCTADAFUCATABYMEANSOFDISSECTINGPARENTSWASSTUDIED．THERESULTSSHOWEDTHATTHESUITABLECONDITIONSFORARTIFICIALINSEMINATIONINAMMONIASEAWATEROF28．6“CWHILESELECTINGEGGSANDSPERMATOZOAFROMTHEMATUREPARENTSDISSECTEDWTJRE；RATIOOFFEMALEANDMALE5191，THEOPTIMUMRATIO7L；PH8．59．0；SALINITY3035，THEOPTIMUMSALINITY30．EFFECTOFPARAMETRESOFPULSEELECTROPORATIONSYSTEMOFGENEPULSERIIRFMODULEINCLUDINGTOTALJOLTS，BURSTDURATION，NUMBEROFBURSTSANDFREQUENCYONACTIVATIONRATEANDFERTILIZATIONRATEOFPINCTADAFUCATASPERMATOZOAELECTROPORATEDWASSTUDIED．CHOOSINGTHATBOTHTHEACTIVATIONRATEANDFERTILIZATIONOFTHESPERMATOZOAELECTROPORATEDWEREABOUT50％ASSTANDARD,THEOPTIMUMPARAMETERSOFELECTROPORATIONINTRANSGENEOFPINCTADAFUCATAUSINGGENEPULSERIIRFMODULEWEREDETERMINED．WHICHWEREBELOW．TOTALVOLTSWAS200V,BURSTDURATIONWAS1．ORES，NUMBEROFBURSTSWAS5ANDFREQUENCYWAS30K50KHZ．FOREIGNDNAWASINTRODUCEDINTOTHEGENOMEOFPINCTADAFUCATABYELECTROPORATIONOFSPERMWITHPULSEELECTROPORATIONSYSTEMOFBAEKON2000MODEL．SPERMATOZOAOFPINCTADA扣加以WASINCUBATEDWITHLINEAR“ALLFISH”GROWTHHORMONEGENEPCAGCGHFOR30MIN．THENMATUREEGGSWEREFERTILIZEDIAVITROWITHTHESPERMCELLSTREATEDWITHELECTROPORATIONATLOKVAND2。PULSESFORTENCYCLES．DNAWASⅡ