不同基質(zhì)配比對青辛速釋貼質(zhì)量的影響

1、表9HP—Innowax柱上姜黃揮發(fā)油GC譜8主峰保留指數(shù)數(shù)據(jù)Table9RetentionindicesofeightmainpeaksinGCchromatogramsofessentialoilinC.longaobtainedwithHP—Innowax柱前壓kPa升溫速率(℃min1)保留指數(shù)12345678804158517231729177617822193225722727041590172517311778178421

2、97226122778031572171617211769177621852247226370315771719172417721778218822502266平均相對保留值15811721172617741780219122542270RSD%0.510.230.260.230.210.240.280.28圖2姜黃揮發(fā)油HP—Innowax柱GC程序升溫測定圖譜Fig.2GCchromatogramofessentialoilinC.

3、longaobtainedwithHP—InnowaxReferences:[1]RegulationsftheEstablishmentoftheFingerprintSpectraStardoftheTCMInjections(Draft)(中藥注射劑指紋圖譜研究的技術(shù)要求(暫行)[S].2000.[2]HongXKWangZHGuoJXetal.ThefingerprintspectrumanalysisofGCrelativer

4、etentionvaluesfessentialoilof19speciesofBupleurumGenus[J].ActaPharmSin(藥學(xué)學(xué)報)198823(11):839845.[3]KovatsE.GaschromatographicacterizationofganiccompoundsⅠ.Retentionindexesofaliphatichalidesalcoholsaldehydesketones[J].HelvC

5、himActa195841:1915.[4]VanDenDoolHKratzPD.Ageneralizationoftheretentionindexsystemincludinglineartemperatureprogrammedgasliquidpartitionchromatography[J].JChromatogr196311:463.不同基質(zhì)配比對青辛速釋貼質(zhì)量的影響鄭國1羅光亮2彭榮珍1?(1.暨南大學(xué)醫(yī)學(xué)院第六附屬醫(yī)院

6、江門市五邑中醫(yī)院廣東江門5290312.深圳市福田區(qū)中醫(yī)院廣東深圳518034)摘要:目的選擇以明膠、西黃蓍膠、甘油、聚丙烯酸鈉和青黛制備青辛速釋貼的最佳基質(zhì)配比。方法應(yīng)用正交試驗法分別以粘度、軟硬度、清涼感和刺痛感為衡量指標綜合評定不同基質(zhì)配比下青辛速釋貼的質(zhì)量。結(jié)果青辛速釋貼的最佳基質(zhì)配比為明膠∶西黃蓍膠∶甘油∶聚丙烯酸鈉∶青黛(8.0∶3.5∶20∶1.3∶3.0)。結(jié)論采用青辛速釋貼的最佳基質(zhì)配比可制備質(zhì)量較佳的青辛速釋貼。關(guān)鍵

7、詞:青辛速釋貼基質(zhì)正交試驗中圖分類號:R283.6文獻標識碼:B文章編號:02532670(2003)09079703EffectofdifferentproptionofmatrixonqualityofQingxinSushiTieZHENGGuoshen1LUOGuangliang2PENGRongzhen1(1.SixthAffiliatedHospitalWuyiHospitalofTCMMedicalCollegeofJin

8、anUniversityJiangmen529031China2.FutianHospitalofTCMinShenzhenShenzhen518034China)Abstract:ObjectTotheoptimumproptionofmatrixfthepreparationprocessofQingxinSushiTie(QST)bygelatiagumtragacanthglycerolsodiumacylateQingdai.

9、MethodsTheprocesswasstudiedbythogonaltestwithviscositysofthardmeasurementalgefacientsentationtwingesentationasdetectivemarker.ThequalityofQSTwascomprehensivelycomparedbythedifferentproptionofmatrix.ResultsTheoptimumpropt

10、ionofmatrixinQSTwasgelatiagumtyagacanth797中草藥ChineseTraditionalHerbalDrugs第34卷第9期2003年9月?收稿日期:20021101基金項目:廣東省中醫(yī)藥管理局立項課題(099628)作者簡介:鄭國(1964—)廣東江門人副主任藥師主要從事中成藥的生產(chǎn)管理和開發(fā)研究在省級以上的學(xué)術(shù)期刊發(fā)表論文23篇。Tel:(0750)35282883136表2L16(215)正交

11、試驗結(jié)果Table2ResultsofL16(215)thogonaltest序號123456789101112131415DEACDBDCCEBDEDA指標粘度軟硬度清涼感刺痛感綜合評分11111111111111112.00.500.40.253.1521111111222222222.50.500.80.504.3031112222111122222.01.502.00.506.0041112222222211112.52.501

12、.20.256.4551221122112211223.00.500.40.254.1561221122221122114.01.000.80.506.3071222211112222114.01.502.00.508.0081222211221111222.52.501.60.256.8592121212121212222.00.500.80.503.80102121212212121114.01.000.40.255.6511212

13、2121121221113.52.501.20.257.45122122121212112221.51.502.00.505.50132211221122112222.00.500.80.503.80142211221211221114.01.000.40.255.65152212112122121114.52.501.60.258.85162212112211212221.51.502.00.505.50Ⅰ45.242.343.746

14、.845.246.145.245.243.644.746.139.248.251.546.3Ⅱ46.249.147.754.646.245.346.746.247.846.745.352.243.239.945.1R1.06.84.07.81.00.802.01.02.62.00.813.05.011.61.2S0.062.891.0019.80.060.040.250.061.100.250.0410.61.568.410.09表3方

15、差分析表Table3Varianceanalysis方差來源離均差平方和自由度均方F值顯著性A2.89012.89010.00P0.05B0.25010.2500.865C19.800119.80068.510P0.01D10.560110.56036.540P0.01E1.56311.5635.408AD8.41018.41029.100P0.01BD0.06310.0630.218CD0.06310.0630.218ED0.0631

16、0.0630.218EC1.10311.1033.817誤差1.44350.288F0.05(15)=6.61F0.01(15)=16.33結(jié)論與討論3.1因素D為水平2時大部分粘度評分為4.0以上可認為因素D是影響粘度的主要因素。因素C為水平2時大部分軟硬度評分為1.5和2.5大部分清涼感的評分在1.2以上可認為因素C是影響軟硬度和清涼感的主要因素。因素E則由于青黛的藥理作用選擇水平2時則產(chǎn)生明顯的刺痛。因此可根據(jù)實際質(zhì)量的考察和醫(yī)患

17、雙方的使用要求軟硬度和清涼感不足時增加甘油的用量藥效不顯著時增加青黛的用量但應(yīng)該注意青黛用量增加時對巴布劑粘性的破壞。由于A與D存在極顯著的交互作用粘度不足時應(yīng)同時增加聚丙烯酸鈉和明膠的用量可大幅度提高粘度。3.2青黛的主要成分為靛玉紅和靛藍均不溶于水有機溶劑會影響巴布劑中水溶性基質(zhì)的作用因此青黛以原粉制劑。初步試驗證明青黛粗粉破壞巴布劑的粘性因此采用貝利微粉機將青黛粉碎為300目以上的微粉微粉化后的青黛產(chǎn)生類似增塑劑的作用故本實驗不另

18、加增塑劑。此外微粉化中藥有利于有效成分的釋放[2]。關(guān)于青辛速釋貼的透皮吸收情況有待進一步的研究不同透皮吸收促進劑對釋藥的影響也有待進一步研究。3.3粘度和軟硬度是《中華人民共和國藥典》2000年版對巴布劑的要求但清涼感有助于祛熱和減輕患兒痛苦輕微的刺痛感則可確認藥物的作用。因此青辛速釋貼的清涼感和刺痛感也列入綜合評分。3.4青辛速釋貼用于兒科臨床治療小兒外感風(fēng)寒、風(fēng)熱表證已初步取得滿意的療效。References:[1]CaiGQZh

19、ouJXuY.StudyonremovingmethodofharmfulsafroleinHerbaAsarivolatileoil[J].ChinTraditPatMed(中成藥)199719(7):37.[2]DuXMGuoQHeY.ComparisonofpharmacodynamicsbetweentraditionalpreparationofChinesepatentmedicinespreparationmadeinul