水稻向地性突變體的生理研究、遺傳分析及相關(guān)基因的分子標(biāo)記定位.pdf

1、四川農(nóng)業(yè)大學(xué)碩士學(xué)位論文水稻向地性突變體的生理研究、遺傳分析及相關(guān)基因的分子標(biāo)記定位姓名：楊芳申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：作物遺傳育種指導(dǎo)教師：汪旭東凌宏清20030501AbstractRice(OryzasativaL)isanimportantworldwidecerealcrop，aswellasamodelplantformolecularbiologyresearchAlthoughriceagravitropismmutant

2、(1azy—rice)wasrepodedin19381ittleresearchhasbeendoneaboutitAtpresent，relatedgenecharacterizatiOnorclonehasnotbeenrepoded，andthenrelatedmolecularmechanismisalsounkownToIearnmolecularmechanismongravitropismofricewehaveobta

3、inedariceagravitropismmutant《lazy2)fromM1populationofindicalineE428，whichWastreatedbyEMSThecharactersofthismutantriceincludedown—growthduringseedlingandlazygrowthhabit(prostrategrowth)sincetiUerandothercharactersarenorma

4、lIthasbeenshownthroughpreliminaryphysiologicresearchthatexogenousphytohormonesandlighthavenotanyeffectontheagravitropismcharacterofthismutantlazy一2，whichhasnesponsetogravitytheresultofgeneticanalysisforlazy一2mutantindica

5、testhatthismutationiscontrolledbyasinglerecessivegeneAndrelatedgenehasbeenmappedbetweentwoSSRmarkeron11chrThemainresultsaresummarizedasfoIlows：1Thephenotypeof、／、廠randlazy一2mutanthasnochangeinMSwithdifferentconcentrations

6、ofexogenousphyfohormonesf24一Dandgibberellin)comparedwithnormalconditionsButthedevelopmentofbothhavebeeninhibitedwithhigherconcentrations，thebothphenotypesa舊notobviousTheresultshasshownthatexogenousphytohormoneshavenoobvi

7、ousaffectionongrowthhabitsofta2mutant2Aftergrowthfor7daysincontinuouslightandcontinuousdarkness，thephenotypesof、／、廠randlazy2mutantarelikeasthoseinnormalconditionsTheresultshasalsoshownthatlighthasnoobviousaffectionongrow

8、thhabitsofla一2mutant3＼／、廠randlazy一2mutantgrowindifferentgravitationalfield(10catedinvertlyandlevelly)Theupperandundergroundorgansof＼^廠rareallabletosensegravityWhereastheupperorgans(stem)oflazy一2mutanthas’tanyresponseongr