哈薩克羊、阿勒泰羊、巴什拜羊遺傳多樣性的AFLP分析.pdf

1、石河子大學(xué)碩士學(xué)位論文哈薩克羊、阿勒泰羊、巴什拜羊遺傳多樣性的AFLP分析姓名：付永申請學(xué)位級別：碩士專業(yè)：動物遺傳育種與繁殖指導(dǎo)教師：王金富20090601AFLPAnalysisofGeneticPolymorphisminKazak、AletaiBashibaiofIndigenousSheepBreedsinXinjiangAbstractXinjiangistheprovinceofChma、)lmeresheepbreeds

2、isthemostlargestandhascomplexgeneticqualityithasbredanumberofcharacteristicslocalvarietiesthatisavaluableassetfordevelopmentandutilizationoftheworld’SanimalresourcesinthelongtermproductionpracticeKazakhstansheephasexcell

3、enttraitssuchaslargebody，prematudtyresistance，andadaptability：AltaysheepisagoodgroupofKazakhstansheepthathavemuchfeatureforexamplelargebodyandfastgrowthoflambandfastfatandhighfatmeatproduction；Bashibaisheepalsohasmanyfea

4、turessuchasprematurity，resistantcoarsefeed，strongadaptabilityandbrownredhairandmultipileanddeadhairlessdryandSOonTheselocalvarietiesistheimportantspeciesthatisusedtOprotectthebiologicaldiversityanditistoachievethebasisof

5、sustainabledevelopmentofsheep—raisinginChinaInthisstlldy，forthefirsttimeuseAFLPmarkerstoclarifypolymorphismofXinjiangbreedsresourcesamongKazaksheep，Altaysheep，andBashibaisheepfromthemolecularlevel，itwillprovideascientifi

6、cbasisforconservationandrationalforthputtingofthelocalspeciesUsingoftheAFLPmarkers，thisexperimenthasaccomplishedgeneticanalysistoKazakhsheep，BashibaisheepAltaysheepinXinjiang，hascountedthepolymorphicbandsthateachprimerco

7、mbinationsdetectedinallvarieties：hascalculatedgeneticsimilaritycoefficientgeneticdistanceofthreelocalvarietiessheep，andbased011that，constructedtheUPGMAclusterdiagramTheresultsareasfollows：1Comparisonoftheboilingwaterbath

8、，Millersaltingoutmethod，CTABmethodandphenolextractionimprovedfourkindsofsheepgenomicDNAextractionmethod，whichextractedinboilingwaterbathforpurityandconcentrationofDNAWassignificantlylowerthantheotherthreekindsofmethodsPh

9、enolextractionimprovedwimMillersaltingoutmethodCTABextractionmethodofDNAcomparedtothepurityandconcentrationoftheproductofasignificantdifference(p005)，suitableforAFLPmolecularmarkersofDNAExperimentalrequesttemplate2Thisst

10、udyhadgonethroughanumberofrepeatedlyscreeningtoatotalof64MseIandEcoRIprimercombinations，inwhichobtained11primercombinationswithmorebandsandhigherpercentageofpolymorphism，richerbandingpattern，clearerstrips(E01／M01，E01／M07

11、，E02／M03，E04／M01，E05／M05，E05／M08，E06／M02，E06／M04，E06／M05，E07／M03，E07／M08)Atotalof527stripsinthethreelocalsheepvarieties，onaverage，4791stripswereamplifiedperprimerscombination，thescopeofthechangesisthe34to61，including46po