蟲草菌抗逆性及其對家蠶致病性的研究.pdf

1、中國農業(yè)大學碩士學位論文蟲草菌抗逆性及其對家蠶致病性的研究姓名：張軍申請學位級別：碩士專業(yè)：農業(yè)昆蟲與害蟲防治指導教師：陳建新宋敦倫20030501AbstractInthisthesisthelatestresearchstatusaboutCordyeepswerereviewed；effectsofultravioletradiationtemperatureandpHongerminationofCordyeepsconidio

2、phorewerestudied；physiologicalandbiochemicalchangesofsilkwormBombyxmoriduringCordycepsmilitarisinfectionwerestudiedByhistologicalsectionobservationthehistopathologychangesoftheinfectedlarvaewereillustratedTheresultswerea

3、sfollows：Studiesontheeffectsofultravioletradiation，temperatureandpHongerminationofCordycepsconidiophoreshowthatthegerminationofCordyeepsmilitarisconidiophorewasdrasticallyeffectedbytheultravioletradiation，andthegerminati

4、onofCordycepssinensisconidiophorewasdrasticallyeffectedbydifferenttemperaturesThepHvaluesfiredtothegerminationofCordycepsconidiophoreareunder7andtheoptimumrangeis4～5Whenthe5“instarsilkwormlarvaewereinfectedbyCordycepsmil

5、itarisconidiophore，thecontentofhaemolymphcarbohydrates，trehalose，proteinandglyceridedifferentlydecreasedandthedecreaseofhaemolymphglyceridewasmostobviousTheactivityofhaemolymphtrehalasealsodecreasedininfectedsilkwormThea

6、ctivityofhaemolymphSOD，PODandCATwerechangedafterthesilkwormwereinfectedFrom441841U／mLto601255U／mL，theactivityofSODincreasedmostsignificantlyinfourdaysTheresultsofhistologicalsectionobservationsimpliedthat，inthefirsttwoda

7、ys，thehaemocytesofthe5“instarsilkwormlarvaemighttakeadefenseresponseaftertheywereinfectedbyCordycepsmilitarisconidiophoreandalargeamountofhyphaecall’tbefoundThedefensecoursecouldbefinishedin2daysThen，hyphaeandconidiophor

8、ewerefoundinthelarvalintegumentTherewerelittlehyphaeinotherorgans，but，exceptformalpighiantubules，mostoforgansweredisintegratedandvacuolatedWecanconcludethatthetoxin，whichwassecretedbyCordycepsmilitaris，maycausethedeathof