29853.海洋酵母普魯蘭類酵母(aureobasidiumpullulans)鐵載體的研究

1、中國海洋大學(xué)碩士學(xué)位論文海洋酵母普魯蘭類酵母（Aureobasidiumpullulans）鐵載體的研究姓名：王偉琳申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：海洋生物學(xué)指導(dǎo)教師：池振明20090601Thesmdiesonsiderophoreproducedbytllemarilleyeast4擾71PD6口sZ西擾，規(guī)矽“刀擾勵(lì)萬sHN624擾陀DD口SZdZ擾，規(guī)矽“ZZ擾Z口萬Sfi上NOZAbs訂actoVer300yeaSts仃ainsiso

2、latedfromdi丘erentmariIleen、，的IⅡnen：tSwerescreenedformeirabilit)，toproducesiderophoreAmongmem，atotalof14yeaStg昀ills、ⅣerefouIldtoproducesiderophore，aIld也eyeaStsn面nHN62wbjchwaSidemifiedtobe么擾陀D6礎(chǔ)f旃”聊p“刀砧脅力swaSfbulldtoproduc

3、ehighleVelofthesiderophoren坨optimalmediumcomponemsa11dgrowmcond“ionsforsiderophoreproductionbys仃aillHN62werestudiedusing“one—v撕able—atat硫e’，methodneresultsSho、礙dt11atmeoptimmmediumcoInponentsfor也e11ighestsiderophoreyield

4、bysn嘶nHN62weresllcrose3O％(w，v)，NH4N0303％(w，v)，K2HP04O3％(w／V)，citricaCid01％(w／V)，MgS04O008％(w，v)，ZnS04OO002％0，／v)，1OmMLomitl：1inea11d吐leoptiInalgrowmconditions、ⅣereShahngSpeed200rpm，“tialpH62，tempera：tlⅡle28℃Underthesecon

5、ditioIlS，mesiderophoreinⅡleliquidmediumreachedmelligheStyields11mg／n兒aRer120hoffennentationInthiss缸1y，wefound虹latadditionofLornjtbineiIltlleproduCtionmedi啪coulde11llaIlcethesiderophoreproductionbyHN62，whereaLs也esupplemen

6、tofexcessFe3intheproductionmediumcoulddecreasethesiderophorepro(1uCtionIntllemolecularleVel，也eexpressionofnlegeneofhydroxylasewaSalsepressedin也epresenceofFe3，111eexpressionoftllegenewasstimulatedi11舭presenceofLOmitmneWed

7、etectedantimicrobialactiVi夠ofthecmdesiderophoreproduCedbystri王illHN62byt11eOxfordcupsa11dc010ny—co硼tingTheresultsshowed吐1atthecmdesiderophorehadclear砌ibacterialactiVi夠againStt11e似omarine越malpa_mogeIlS，Ⅳ口，zg“f，，(礦“聊andⅣp日

8、，口Jiz口P聊Db嘭fc。岱andtheconlIIlonbacterium丑s“6f玎括HoweVer，thecmdesiderophorehadnoaIltimicrobiaJactiVit)ragainstt11emostco瑚monbacteria，E∞Zf，S口“陀姍，the彪6fc“印f如把WCYtwo劬gi彳嘲羅rand尸P，z耙肋淞SpnleresultSalsoshowedmattheheatI甜siderophor