45293.異色瓢蟲harmoniaaxyridis腦內5ht及其合成酶tph的免疫組織化學定位研究

1、學校代碼：三Q2鯉分類號：逝④東秘啼葒女莩碩士學位論文研究生學號：!叢22Q3Q塹密級：玉異色瓢蟲凰口刪D刀缸以砂r洶s腦內5一HT及其合成酶TPH的免刃。氫織化掌定位習院ImmunohistochemicallocalizationOf5一HTanditssynthesizingenzymeTPHinthebrainofthebeetle上砌，J豫口尼缸證篾“’f扮作者：田喜梅指導教師：暴學祥教授學科專業(yè)：動物學研究方向：昆蟲腦神經生

2、物學學位類型：學歷碩士東北師范大學學位評定委員會2006年5月Abs觚ctAppliedrabbit髓蛀‘5HTandmouSemonoclonalantiTPHa碰serathelocal溉ionof5一Hr卸ditssyn廿他sjzing鋤zyme11PHinthevis嚙lsysb鋤，midbm吐andsuboesopllagcalgallglionofmebeetle覷玎冊徹蛔醚M配如(CoIe咖：Cocci∞md神)哪snld

3、iedbystrep州dinPem)【idaseim刪mohi咖ch鋤ical礎mod∞Col蝴Pa托伍n鋤beddedserialsecdonsAnoVemlIob辯rv鰣onrevealsthatthebfa缸strucnI∞ofthebectlcisrel砌vclydisthlct髓eopticlobcisdevc王opcd，and血讎e)【ists1d眥apl砷e；也co啦劬哪!eiscomplexTheglomeruli蛐n】

4、ctllreofar魄lmallobeisnotcl蹈sicalAccc眥I主llgl弘恤emushroombodycalyceshavemeager，鋤dthelobesarerela虹Velys蛐pleei也crThece：噸：alc∞叩l怯ispronlinentThebeeueshowednotable5一HT1ikeinlIllunoreacti嘶血DlIgholItn把wholek曲，wllichwasalso咖nglyla

5、belledby皿Han曲ody，wi也t1“pat蛔mma扛斌雌衄of5_HT5一HT1il(eposjtive舶erswercresolvedinauar髓sof也e砌lill鋤ong刪ch也e1鋤inaandsuboesop：hagealg鋤91ionshowedthemostr鼬鉞ki直bledarkbrownimmllIlostaillingW11ile，allm司0rneumpilregions，ingeneral，show

6、edlowlevclbac：kgroundafbr即Hi1IlmunostaiⅡiI培for口Hmainlye婦g曲gi11cytoplasmn坨mⅢIbcrof5HT_l婦immunoreactiveneuronswasrelativelysmallbutpossessed黝枷ve謝co鼯盯borizanO王lsMostof5HTlikeiInmulloreactiVeneuronswcret鋤gemialncur∞sandbilat

7、erallysymmetricaldismuted111eneurocyte帥e，DuInber，dis伍b嚦on孔dprqje鋤nof硎mneurons、veresimilartotllatof5HTh1the、，isualSystem，5一H7r，TPHm【eimⅡl阻m酆tivenellrons(ca35perhemisp舊∞weremaimysitu種edbe帆een1heo州clobe鋤dlateralprotoccrdnm，

8、theneuritesofw11icheitllerdescendediIltotheo皿cneuropilsor勰c∞dednothepmtocerebmm，thuspossiblynegra廿ngtlleopticlobes缸Ldoth盯br血re西ons111mcprotocerebnm，5H咖Hlikei舢unoreactiveneⅢons(ca。25)m蛐diSmbuteddorsalto也eprotocerebnmbesid

9、emeKenyoncells1heiraxonsd曲cnyprojectedinto也eprotocerebnⅡnaSwellastheopticlobeOmylsin西e5HT廠rPH—likeimmunoreactiveint眥1eumnwaslocalizednear也edorSalantennallobe，conldbu血gto瑚Dstimmunorcactivi鑼in吐岵antemllobeInthesuboesophagea

10、lganglioⅡ，5一Hr腫Hl讓rei啪mlorcactivene啪ns(c亂15)mailllydis蛹butedin廿1elateral姐dventm—laterala∞a，wimprocessespmjectingiIltothesuboesophagealgan西ionneuropilThecollectiveresIlltsindicatcd恤t5HTplaysa苴1importantroleiIlthevisuali11

11、fo徹丑tionprocessingandmo叫砒esdi啪alrhytllm刪yviamedist妣taIldextensivepatt鋤hlsomespeci矗careas，5一HTisc010calizedandmaysenreasco缸aIlsmiCterswiⅡ1GABA111addition，5一HTcontentill血eVisualsyst鼬isprobablycloselyrclatedt0tllephysiologi