25996.擬南芥器官發(fā)生過程中生長素濃度梯度作用的初步研究

1、蘭州大學碩士學位論文擬南芥器官發(fā)生過程中生長素濃度梯度作用的初步研究姓名：哈飛申請學位級別：碩士專業(yè)：細胞生物學指導教師：劉恒20100501蘭州大學碩士研究生學位論文AbstractPlants，comparedtoanimals，showallamazingadaptabilityandplasticityintheirdevelopmentThiSismainlydependentontheabilityofplantscanfo

2、rmnewpostembryonicorgans，suchaslateralroots，leaves，branches，flowersandSOonThroughthecoordinationofeelldivisionanddifferentiationfoundereellsCanformtoorganprimordialandeventuallydevelopintothematureorgansOrganformationinA

3、rabidopsisinvolvesdynamicgradientsofthesignalingmoleculeauxinwithmaximaattheprimordiatipsUsingthemodelplantArabidopsis，enrichourunderstandingonthefunctionsofauxininplantorganogenesis，thereforeexploredthefunctionofauxinal

4、SObecometooneofthemostpopularresearchareasindevelopmentalbiologyThispaperArabidopsisasthematerial，throughresearchtheroleofauxininpostembryoorganogenesisandfoundthattheformationoflateralrootprimordiaalongwiththeestablishm

5、entofauxingradients，andtheformationofauxingradientmadelateralrootprimordiahasallindependentmeristematiccapacityTheformationofauxingradientrelatedwiththeauxintransportandresponseproteins，andthemutationsofauxinrelatedgener

6、educedthelateralrootsnumbersindifferentlevels，inadditiontoauxinexportprotein——PINfamilyintheprocessoflateralrootprimordialformationandtheirdifferentorientationindicatetheirdifferenteffectsontheauxingradientformationMeanw

7、hile，inordertofurtherstudythefunctionsofauxingradientinplantorganogenesis，usingtheboundarygeneCUc3promotermadetheauxinsynthetaseexpressioninorganinitiationbordeLconstructionoftransgenicplantsaimedthatthroughinterferencee

8、ndogenousauxingradientinorganinitiationprocessandstudytheeffectofauxingradientonplantorganogenesisOurresultsshowthattransgenicplantswithmanyphenotypes，suchasleafcurling，leafareareduced，acceleratedgrowth，lateralrootsandbr

9、anchesreduced，hypocotylslongerstemsandleaveswiththestartingpositionabnormalandsomeabortionSomeofthesephenotypesiSsimilartothereportedfunctionofauxinsynthetaseyucc6gainoffunctionmutantphenotype，andtheotherpartissimilartoc

10、uc3mutantphenotypes，moreovertheremainingphenotypesweremainlyduetointerferencewithauxingradientintheprocessoflateralorganinitiationcausedTheseresultssuggestthatauxingradientplayacrucialroleintheformationandmaintenanceofth