豬垂體轉(zhuǎn)錄因子（POU1F1）基因部分序列變異分析及其與生長性能的相關(guān)性研究.pdf

1、揚(yáng)州大學(xué)碩士學(xué)位論文豬垂體轉(zhuǎn)錄因子（POU1F1）基因部分序列變異分析及其與生長性能的相關(guān)性研究姓名：滕勇申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：動(dòng)物營養(yǎng)與飼料科學(xué)指導(dǎo)教師：經(jīng)榮斌20040501VariationsinPartialRegionofPorcinePOUIFIGeneandItsRelationwithGrowthPorformanceN①candidate：YongTengSupervisor：ProfRongbinJingColle

2、geofAnimalScienceandTechnologyYangzhouUniversityYangzhouJiangsu，China225009AbstractThisresearchwasconductedtoanalysethesequencevariationsinpartialregionofpigPoUlFjgene，includingexon4exon5，exon6andintron3，byusingPCR—RFLP，

3、PCRSSCPandsequencingtechnequeSixpigbreeds(Landrace，Duroc，Yorkshire，Jiangquhai，MeishanandXiang)wereselectedasexperimentalanimalAlsoacommercialpigpopulationgrowthperformancewascorrelatedwiththepolymorphismofintron3Theresul

4、tswereshownasfollows：1Nopolymorphismwasfoundinexon5andexon6ofPOUIFlgeneinallbreedsApointmutationbutaminoacidsubstitutewasidentifiedinexon4Allele(G，H)frequenciesinthisrestrictionsiteindifferentpigbreedsdistributedsignific

5、antlywithhighHallelefrequencyinforeignmeatpigsandlowinChineselocalbreedsWhileamonghuman，pig，mouseandcowPOUHDandPOUsDencodedbyexon4werehi曲conservation2AMspIpolymorphismmportedpreviouslywasidentifiedinintron3Therewasasigni

6、ficantdifferenceofallelefrequenciesinthissiteamongdifferentbreedsaswellTheDallelefrequencywashighinforeignmeatpigs，whilelowinChineselocalfatpigs3Accordingtoanalysisofcorrelationbetweengrowthperformanceandgenotypesincomme

7、rcialpigs，bodyweight(expectbirthweight)anddailygainindifferentstagesbetweenDDgenotypeandothergenotypesdifferedsignificantly(PO05)orextremelysignificantly(P001)，respectively4Bycomparinghomologousanalysisofnucleotidesequen