22717.奶牛乳腺上皮細(xì)胞體外培養(yǎng)體系的建立及其應(yīng)用

1、新疆農(nóng)業(yè)大學(xué)碩士學(xué)位論文奶牛乳腺上皮細(xì)胞體外培養(yǎng)體系的建立及其應(yīng)用姓名：崔立莉申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：王加啟羅淑萍20060501英文摘要(Abs”act)Culturcsystemw鷦establishedtostudytlleeff~ectoflinoleicacidrLA)andvitaminA(retinoicacid，RA)ontheprolifemtionanddi￡fcrentiationof

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7、bascmentmembraneuntil30mdavSTheresultsshowedthatmilkcanaltenlativelvbeane疵ctivesourceforisolatingandcllltu血gofBMECThesccondpartestabIishedthecultllrcsystemofBM匣CFirstly，tlleceUbiol0西calpmpcnieswercobservedandinvestigated

8、丘omtheccllplaⅡtingsurvivalefficiency，cenpopulationdoublingtime鋤dcellgrowthcurveTheresultsshowedthattheBMEC譬鄧唧swelIandproliferat鷦vi90岫uslyiIlUliscunurcsystemTherew蠲asi刪丘咖tdi飪brenceinmediameterofcellsisolatedf幻mma衄齟ym孤dexp

9、lantandboviIlemilk(P00011Theresultsforim蛐l(wèi)Ⅱ0chemicalidenti丘cationofcvtokera血18，也eSDS—PAGEandamplificationofa毫1casein鼬neiⅡBMECindicatedthataccomplishedceUsareBMECThethirdpanexalniIledthee婦EcctofLA0rRA0nBM[ECMITassaVw舾used

10、tocx眥iⅡetheactivityofBMECh跏v豇rDcxperiment，tlleBMECwereculturedi11DM[EM／F】2containi雎0(∞mr01)、10、50、100、200、400uML～and0(con叻I)、O15、15、15、150、1500、15000n鮒∞lRATheactiv蛔o(hù)fBMECwifh400uMLAor15n∥ⅢlRAwcresi罡皿ificantlylowerthantha