16928.華北藍(lán)盆花組織培養(yǎng)及其黃酮素類化合物成分研究

1、摘要華北藍(lán)盆花是川續(xù)斷科(Dipsacaceae)“藍(lán)盆花屬(Scabiosa)植物，具有較好的抗炎、解熱、抗氧化等作用，是重要的蒙藥藥材之一。本論文以華北藍(lán)盆花無菌苗的葉片和葉柄作為外植體，探討了不同的植物生長(zhǎng)調(diào)節(jié)劑及不同濃度的配比對(duì)愈傷組織誘導(dǎo)、不定芽分化、生根的誘導(dǎo)效應(yīng)，建立起了華北藍(lán)盆花的組織培養(yǎng)再生體系。對(duì)野生植株、愈傷組織和再生植株的染色體數(shù)目進(jìn)行了觀察。采用紫外分光光度法對(duì)野生植株、愈傷組織和再生植株的黃酮類化合物進(jìn)行分析

2、，并測(cè)定了其清除DPPH自由基的能力。本研究為華北藍(lán)盆花的進(jìn)一步開發(fā)與利用提供了理論依據(jù)。主要研究結(jié)果如下：1、以華北藍(lán)盆花的葉片和葉柄作為外植體，誘導(dǎo)愈傷組織的較適宜培養(yǎng)基為MSTDZ20mg／L2，4一D05mg／L的培養(yǎng)基，葉片誘導(dǎo)效果最好，誘導(dǎo)率達(dá)到9405％，且愈傷組織狀態(tài)較好，呈淡黃色結(jié)構(gòu)疏松。2、以華北藍(lán)盆花的葉片和葉柄作為外植體，誘導(dǎo)不定芽的較適宜培養(yǎng)基為MS6一BA40mg／L，葉片誘導(dǎo)率為863％；MS6一BA20m

3、g／L，葉柄誘導(dǎo)率為8333％。棚T勝cT㈣嬲HuabeiScabiosa，Scabiosatschiliens／sGrunning，isaplantinthefamilvofDipsacaceaeTheflower，whichhasaneffectofanti—inflammatory，antipyreticandanti—oxidation，hasbeenusedasanherbmedicineinhistoryandhadwide

4、lybeenusedinMongolianandChinaInthispaper，ScabiosatschiliensisGrunningleavesandpetiolewereusedasexplants，andwediscussedtheinductiveeffectofcallus，adventitiousbudandrootindifferentplantgrowthregulatorsanddifferentconcentra

5、tioncombinationcultureThesystemofrapidregenerationandpropagationoftissuecultureofScabiosatschiliensisGrunningwasestablishedThechromosomenumberofthewildplant，callusandtheregenerationplantshasbeenobservedThecontentofflavon

6、oidsfromwildplant，callusandregenerationplantwereanalysisedbyultravioletspectrophotometry，andfreeDPPHradicalsabilityweredeterminedbyELIASAAlltheresultssetupthefoundationforfutureexploitationandresearchofScabiosatschiliens

7、isGrunningThemainexperimentalresultsareasfollows：1ScabiosatschiliensisGrunningleavesandpetiolewereusedasexplantsthebestmediumofcallusinductionwasMSmediumwith20mg／LTDZand05mg／L2，4一Dbyleaves，theinductionratewas9405％andthec

8、alluswerefaintyellowandlooseingoodcondition2Scabiosatschi／iensisGrunningleavesandpetiolewereusedasexplantsthebestmediumofadventitiousbudinductionwasMSmediumwith40mg／L6BAbyleaveswhichhas843％ofinductionrate，andMSmediumwith