低聚糖激發(fā)子誘導(dǎo)楊樹細(xì)胞抗病機(jī)制的研究.pdf

1、西北農(nóng)林科技大學(xué)碩士學(xué)位論文低聚糖激發(fā)子誘導(dǎo)楊樹細(xì)胞抗病機(jī)制的研究姓名：周自云申請學(xué)位級別：碩士專業(yè)：植物學(xué)指導(dǎo)教師：胡景江張錫梅20040601DiseaseResistanceofPopulustomentosaCellInducedbvoli90saccharideElicitorsAbstractThephenomenathataplantshowspartialorsystemicresistancetOsomeorganic

2、，phy’sicalorchemicalfactorscallsinductionresistanceThefactorcausingtheinductionresistanceinplantscallselicitorInductionresistanceisanimmunizationinorganismOligosaccharideisakindofbiologicalactivemolecularthatCanmodulatet

3、hegrowthanddevelopmentofplantsAlbersheim，anAmericanresearcher，discoveredthatoligosaccharidescouldinduceresistanceinplantsatmiddleof1970。S。Sincethen，itbecomesahotpointinbiologicalresearchfieldThePopulustomentosacalluswasr

4、educedbythreeelicitors，whichwereproducedbyDothiorellagregariaandchitinInordertoobtainthebestelictor，thechangesofPAL，chitinase，0N—acetyle—D—glucosaminidase，SOD，POD，CATPPO，HRGPandchlorogenicacidweretestedThereasonthatcause

5、stheinductionresistanceinplantwasalsodiscussedTheoptimalconcentrationoftheelicitorI，whichwasproducedbyDothiorellagregaria，correspondingto21ug／mlsaccharideThemostsuitablemethodforproducingelicitor11washydrolyzingtheelicit

6、orIin10％(v／v)lm01／LHClfor30minTheelicitorlllwasmadefromchito—chitinbydissolvingthechitininl：30(w／v)2％aceticacidandhydrolyzingthesolutionin1：03(v／v)3％H20275℃for3hSOD，POD，CATandPPOcouldbeinducedbythethreeelicitorsInducedby

7、thethreeelicitors，themaximumofCATappearedat2hourlaterSODatthe25hourlater，PPOatthe35hourlaterandPODatthe6～12hourlaterChitinase，BN—acetyleDglucosaminidase，HRGPPALandchlorogenicacidcouldbeinducedbythethreeelicitorsChitinase

8、andBN—acetyleDglucosaminidasereachedthemaximumat4hourlaterPALandchlorogenicacidreachedthemaximumat48hourlaterandthecontentofHRGPwasincreasingafterinducingKeywords：Oligosaccharide，Elicitor，Inductionresistance，Populustorae