梅花重瓣性F-,1-作圖群體構(gòu)建及分子標記初步分析.pdf

1、學餃代碼：10504分類號密級V8063’S梅花重瓣性F。作圖群體構(gòu)建及分子標記初步分析ConstructionofF1MappingPopulationandPreliminaryAnalysisofMolecularMarkersofDoubleFlowerTraitofMeiFlower(Prunusmume)研究生姓名：張金波PostgraduateZhaJlgJinbo指導老師：包滿珠SupervisorBaoMaozhu專業(yè)

2、：園林植物與觀賞園藝SpecialtyLandscapeplantsandornamentalhorticulture研究方向：園林植物遺傳育種與生物技術(shù)SubjectLandscapeplantsgenetics＆breedingandbiotechnology淪文報告提交日期：2004年7月學位授予同期：年月日華中農(nóng)業(yè)人學2004屆顱I：論史ConstructionofF1MappingPopulationandPreliminar

3、yAnalysisofMolecularMarkersofDoubleFlowerTraitofMeiFlower(Prunusmume)AbstractMejflower(PruusmumeSiebctZucc)isoneofthetenfamoustraditionalflowersinChinafavoredbyChinesepeopleAmongMeiflowercultivars，thedoubleflowercultivar

4、spossessingornamentalvalueplayanimportantroleInthestudy，thefeasibilityofconstructingamoleculargeneticmapusing“doublepseudotestcross”strategywasdiscussedthrou【ghestablishingaFIpopulationTofindmarkerslinkedtothetargetchara

5、cterswealsodosomeresearchapplyingBSAmethodThemainresultsofresearchareasfclllows：AF1populationwith33jndividualswasconstructedfromthecrossPmtlme‘XueMei’(singleflower，singlepistil)andPmume‘FenpiGongfen’(doubleflowermulti—pi

6、stil)TheRAPDmarkerssegregatingina1：1or3：1ratioaccountfor2857％oftheIotalRAPDmarkersThereforeitisfeasibletouse“doublepseudotestcross”strategytoconstructmoleculargeneticmapsofPrunusmumeTheSSR，ISSRreactionsystemswereestablis

7、hedAndtheprobabilityofMADSboxlikegenesexistinginMe／flowerwasprimarilytestifiedbasedonSSRIcsultParentagedeterminationof27individualsfromtheFlprogeny‘XueMei’‘FenpiGongfen’byRAPDmarkerswasperformedand23ofthemarehybridsThehe

8、terozygosityof‘XueMei’and‘FenpiGongfen’is2273％and2857％respectivelybasedonthesegregationpatternsof28RAPDmarkersinF1populationUsingBSAmethodbyRAPDmarkers，amarker，S1091500，associatedtothetargettraitswasfoundKeywords：Meiflow