福美雙對體外培養(yǎng)的肉雞脛骨生長板軟骨細(xì)胞生長指標(biāo)的影響和Matrilin-3的基因表達(dá)變化研究.pdf

1、華中農(nóng)業(yè)大學(xué)碩士學(xué)位論文福美雙對體外培養(yǎng)的肉雞脛骨生長板軟骨細(xì)胞生長指標(biāo)的影響和Matrilin3的基因表達(dá)變化研究姓名：張艷紅申請學(xué)位級別：碩士專業(yè)：臨床獸醫(yī)學(xué)指導(dǎo)教師：李家奎20080601福美雙對體外培養(yǎng)的肉雞脛骨生長板軟骨細(xì)胞生化捂際的影響和Matrilin3的基因表達(dá)變化研究AbstractTibialdyschondroplasiaisacartilagemetabolicdiseasewhichiswidespreadin

2、poultryindustrywithhighincidencesinyoungbroilers，layingchickensandturkeysTheTDchickensareclinicallycharacterizedbymentaldepressionreducedappetite，grovelingonthegroundusuallydyskinesiaandhavedifficultytostand，sometimeswal

3、king、ⅣimwingssupportedonthefloorwhenseriouslyTheTDepiphysealgrowthplatebecomesthick，sometimesformsopaqueuncalcifiedandunvascularwedgesh印edcartilageextendingfromepiphysealtometaphysealTibialdyschondroplasiaCannotonlyreduc

4、echicken’Sdiseaseresistance，performacetraitandchickenquality；butalsoinducechestcystisandcariousosteitis，andc娥greatlosstopoultyindustryBasedonSUCCesSoftibialdyschondroplasiaintroducedbythiraminvitrol，healthybroilergrowthp

5、latechondrocyteswereculturedinvitroltostudytheeffectofthiramonalkalinephosphatase，acidphosphataseandnitricoxidesynthaseandexpressionofMatrilin3generelatedtobonemineralizationThethiramconcentrationsindifferentgroupsare(o1

6、、I、3、5pm01)After72h，resultsareassayedTheresultshowedthatcomparedwithcontrolgroup，theactivitiesofalkalinephosphataseandnitricoxidesynthaseincreased嬲theconcentrationsofthiramincreasedThesemi—quantitativeRTPCRresultshowedth

7、eexpressionofMatrilin3hadadownregulation嬲theconcentrationsincreasedThiramaffectedenzymesactivitiesrelatedtodevelopmentoftibialchondrocytesandreducedtheexpressionofmatrilin3gene，whichistheetiologyoftibialdyschondroplasiac