氟化鈉對體外培養(yǎng)的人黑素瘤細(xì)胞株A375增殖活性影響的研究.pdf

1、遵義醫(yī)學(xué)院碩士學(xué)位論文氟化鈉對體外培養(yǎng)的人黑素瘤細(xì)胞株A375增殖活性影響的研究姓名：趙敏申請學(xué)位級別：碩士專業(yè)：皮膚病與性病學(xué)指導(dǎo)教師：張信江20100501EffectofFluorinewithDifferentConcentrationsonProliferativeActivityofHumanMalignantMelanomaCellLineA375inVitroAbstractObjective：Tostudytheeff

2、ectofdifferentconcentrationsofsodiumfluclrideonproliferativeactivityofthehumanmalignantmelanomacelllineA375(A375cellline)invitroandexplorethetreatmentofNaFonhumanmalignantmelanoma(MM)Methods：Aftercellswereculturedwithvar

3、iousconcentrations(10、100、500、1000、5000、10000、20000and40000umol／L)sodiumfluoridefor24hoursand48hours，theproliferationofA375celllinewasmeasuredbyMTTmethodThemorphologicalchangesofthecellwereobservedunderInvertedmicroscope

4、Results：NaFpromotedtheproliferationofmelanomacellwhenitWas10umol／LStartedfrom100umol／L，thesurvivalrateofmelanomacellsreducedandtheinhibitionrateenhancedalongwithincreaseddrugconcentrationandtimeextensionHowever10000、2000

5、0and40000umol／LeffectedonA375cellsfor24hs，20000and40000umoFLfor48hsallhadnodifference(礦O05)100and500umol／LshowedaninhibitoryeffectIftheconcentrationofNaFWasabove500umol／L，cytotoxicityappeared：thenumberofcellssignificantl

6、ydecreased，celldetached，changedroundedandsmallernucleusdisappearedandSOonConclusions：FluorideactsdirectlyonA375celllinetostimulatecellproliferationinabiphasicfashion，namelyinhibitsproliferation，evendirectlykillcellsathig