白樺脂酸對人AGS胃腺癌細(xì)胞Hiwi和CyclinB1基因表達(dá)的調(diào)控作用.pdf

1、華中科技大學(xué)碩士學(xué)位論文白樺脂酸對人AGS胃腺癌細(xì)胞Hiwi和CyclinB1基因表達(dá)的調(diào)控作用姓名：楊立靖申請學(xué)位級別：碩士專業(yè)：內(nèi)科學(xué)（血液?。┲笇?dǎo)教師：陳燕201005華中科技大學(xué)碩士學(xué)位論文華中科技大學(xué)碩士學(xué)位論文2EffectofbetulinicacidontheregulationofHiwiCyclinB1inhumangastricadenocarcinomaAGScellsAbstractAim:Toinvestig

2、atetheeffectofbetulinicacid(BA)ontheproliferationapoptosiscellcycleofgastricadenocarcinomacellAGSinvitrotheunderlyingmolecularmechanism.Methods:TheeffectofBAontheproliferationofAGScellswasmeasuredbyusing3(45dimethyl2thia

3、zolyl)25diphenyl2Htetrazolium(MTT)assay.ApoptosiswasanalyzedbyusingAnnexinVfluesceinisothiocyanate(FITC)propidiumiodide(PI)doublelabeledflowcytometry(FCM)Hoechst33258staining.TheinfluenceofBAoncellcycleofAGScellswasteste

4、dbyPIstaining.BothFCMreversetranionPCR(RTPCR)technologieswereappliedtodetecttheexpressionofHiwiCyclinB1.Results:BAexhibitedsignificantcellproliferationinhibitionaswellasitspotencyofinducingapoptosisinAGScellsinvitroinati

5、medosedependentmanner.TheIC50valuef24hwas18.25gmL(95%confidenceinterval:15.16to27.31gmL).CellstreatedwithBAshowedincreasedcellpopulationinG2MphasewithdecreasedSphasepopulation.TheexpressionofHiwiCyclinB1wasdownregulatedi

6、nBAtreatedAGScellsinadosedependentmanner.Conclusion:BApresentedpotenteffectongrowthinhibitionG2McellcyclearrestinductionofapoptosisinAGScellsinvitropossiblyassociatedwiththedownregulationofHiwiitsdownstreamtargetCyclinB1