蛋白質(zhì)組學(xué)方法檢測膀胱移行細(xì)胞癌患者尿液中特異性腫瘤標(biāo)記物.pdf

1、名量大季碩士學(xué)位論文蛋門質(zhì)組學(xué)方法檢洲膀胱移行細(xì)胞癌患者尿液中特異性腫瘤標(biāo)記物指導(dǎo)牧師學(xué)科專業(yè)名稱玲競提采日期靜吏鍪辯日期薔辯蠢自奢主席荊濤DETECTINGSPECIFICTUMoRMARKERSlNURJNEoFTRANSITl0NALCELLCARCINOMA0FBLADDERBYPROTEOMICSMETHoDSABSTRACTObjective：Todetectthedifierentproteinexpressioninth

2、eurineofbladdertransitionalcellcarcinoma(TCC)，pickoutnewtumormarkers，andexplorethecorrelationbetweenthoseproteinsandthepathogenesisofTCCandtheclinicalapplicationofproteomicsmethodsfortheearlynoninvasivediagnosisofTCCMeth

3、ods：Urinesamplesfrom12TCCpatientsandthecontrolgroup(including4patientswithTCCundergoingTUR—BT(transurethralresectionofbladdertumor)orpartialcystectomypreviously4healthyvohmteersand4renaltumorpatients)wereanalyzedusingsur

4、faceenhancedlaserdesorption／ionizationtimeofflightmassspectrometry(SELDI—TOF—MS)ProteinChiptechnologyAllexperimentaldatawereprocessedbycomputertOformthesimulatingmapsandthecontentsofthosedifferentproteinswouldbestatistic

5、allyanalyzedThentheproteinswhichhadbeenpickedoutwouldbeidentifiedinSwiss—ProtproteindatabaseResults：MultipleproteinchangeswerereproduciblydetectedjntheurinesamplesofTCCgroupandControlgroupbyIMAC—Cu3chipincludingsixpotent

6、ialnovelTCCbiomarkersandtwoproteinclustersAmongthoseproteins，2192Da3438Da，4365Da5495Daand8002Dawereup—regulatedinTCCgroupwhile4134Daproteinand3660Da一3830Da，4310Da一5150Daproteinclusterswereup—regulatedinControlgroupCombin

7、ationof血e3438Daand8002DabiomarkersincreasedsignificantlythesensitivityfordetectingTCCto833％withaspecificityof667％The3438Dabiomarkerhadbeenidentifiedas5defensin，andthe431ODa一5150Daproteinclusterwasconsistentwitlgp40protei

8、nintheSwissProtproteindatabaseConclusion：SELDI—TOF—MSProteinChiptechnologyisaquick，easyhandlingandhigh—throughputanalyticmethodThistechnologycandirectlyscreenoutrelativelyspecificpotentialbiomarkersfromtheurineofTCCpatie

9、ntsSOithasabetterclinicalfeasibilityforearlynoninvasivelydiagnosingTCCandwillprovidesomehelpfulinformationforpredictingtheprognosisandstudyingthepathogenesisofTCCPostgraduate：JingTao(Urology)Directedby：ProfDongShengguoPr