德國小蠊乙酰膽堿酯酶的生物化學(xué)性質(zhì)及其抗藥性監(jiān)測研究.pdf

1、分類號密級Y940051單位代碼：10019學(xué)號：B030112弋蛾寰t夭喜’博士學(xué)位論文德國小蠊乙酰膽堿酯酶的生物化學(xué)性質(zhì)及其抗藥性監(jiān)測研究BiochemicalCharacteristicsofAcetylcholinesteraseandMonitoringofInsecticideResistanceintheGermancockroach，Blattellagermanica(L)研究生：錢垃指導(dǎo)教師：直益武熬援合作指導(dǎo)教師：

2、曾曉苴主任醫(yī)|!匝申請學(xué)位門類級別：盤堂譴專業(yè)名稱：盔些昆蟲皇主蟲隨治研究方向：昆蟲壹理堂所在學(xué)院：盔堂生生物拉盔堂院二零零六年六月AbstractTheGermancockroach，Blattellagermaniea(L)，hasbeenailimportanthouseholdpestinChinaformorethentenyearsBecausseofitsaestheticallyunpleasingnatureandpo

3、tentialmedicalandeconomicimportance，mostpeoplehavebecomeinterestedinthisspeciesThebiochemicalcharacteristicsanddevelopmentalchangesofagetylcholinesteraseandmonitoringofinsecticideresistancewereStUdiedintheGel211anCOCkroa

4、chTheresultswereasfollows：BodydistributionandsubcelullardistributionsofAChEfromGermancockroachwereinvestigatedTheresultsshowedthat569％ofAChEactivitywasdistributedintheheadsof丑germanicaThemweresignificantdifferencesofAChE

5、activitycPmicrosomecytosolcellnucleusandcelldebrisThesubstratespeeificitiesofAChEsfromadultmaleswerestudiedusingacetylthiocholineiodide(ATCh)，acetyl(13methyl)thiocholine0VIeTCh)，Spropionylthiocholine(PrTCh)andsbutyrylthi

6、ocholineiodide(BuTCh)[ISsubslrates，respectivelyTherankorderfromthehighestaffinitytothelowestWasMeTChPrl℃hATChBul℃hasindicatedby墨nvaluesinGermancockroachTherankorderforhydrolyzingefficiencyWasATChPrTChMeTChBuTChasindicate

7、dby％“valuesThesubstratespecificityandinhibitioneffectsoffourcarbamateinsecticidesandoneo”ganophosphateinsecticideintheacetylcholinesteruseofGermancockroachwereinvestigated111einhibitiontimecoursecurveshowedthattheAChEofG

8、ermancockroachWassensitivetothetestedinhibitors(eserine，propoxurmethomyl，thiodicarb，DDVP)Thebimolecularreactionconstant(／0indicatedthatAChEfromGermancockroachWasmostsensitivitytoeserineTherankorderofsensitivityWaseserine

9、DDVPDropoxurmethomylthiodicarbThedeterminationofrecoveryrateofinhibition(島)bycarbamatesuggestedthatAChEsfromGermancockroachcanreactivatenaturallythevaluesofk3indicatedthattheAChEinhibitedbythethiodicarbreactivatemodeslow

10、lythantheotherinsecticidesStagedependentofdiehlorvos(DDVP)andpropoxurtoleranceinGermancockroachWasinvestigatedinthelaboratoryusinginsecticidebioassaysdescribedasWHOglassjartestTheresultsshowedtheadultfemalesweremoretoler

11、anttoDDVPandpropoxurthannymphsandadultmalesKT50valuesofDDVPandpropoxurintheadultfemaleswere152minand279min，respectivelyThespecificactivityofAChEfrom1weekoldnymphsWasthehighestamongalltesteddevelopmentalstagesofnymphsanda

12、dultmalesandfemalesThespecificactivityofAChEdecreasedsignificantlywiththeincreaseoftheageofGermancockroachThesensitivit／ofAChEtoDDVPWasthehighestwitha島value，312x104molqmin～，intIelastnymphalstageofGermancockroach(about6we