鈷對(duì)肉羊維生素B-,12-營(yíng)養(yǎng)狀況的影響及其生物學(xué)效應(yīng)研究.pdf

1、中國(guó)農(nóng)業(yè)大學(xué)博士學(xué)位論文鈷對(duì)肉羊維生素B營(yíng)養(yǎng)狀況的影響及其生物學(xué)效應(yīng)研究姓名：王潤(rùn)蓮申請(qǐng)學(xué)位級(jí)別：博士專業(yè)：動(dòng)物營(yíng)養(yǎng)與飼料科學(xué)指導(dǎo)教師：賈志海20060601AbstractFourexperimentswereconductedtoinvestigatetheeffectsofcobaltonvitaminB12statusanditsbiologicalfunctionsinsheepExperiment1：sixteenrumen

2、cannulatedPollDorsetSmalltailedHanwethemwereassignedrandomlytofourtreatmentgroupsandfedthebasaldietsupplied003mgCo／kgDMasformsofcobaltsulphate，cobaltchloride，cobaltacetateandcobaltoxiderespectivelyResultsshowedthatcobalt

3、sulphateandcobaltchlorideresultedinthesimilareffectsofvitaminB12statusoflambs，whilecobaltacetateshowedless(P005)andcobaltoxidetheleasteffectsonproducingvitaminB12Therelativebioavailabilityoffuurformsofcobaltwas100％，943％，

4、846％and77％respectivelyComparedwithothertwoformsofcobalt，cobaltsullphateandcobMtchloridehadamorepositiveinfluenceonproductionofIumenTVFAandmolarproportionofpropionicandthedigestionofEEandfibreHowevercobaltoxideexercisedth

5、eleasteffectsonimprovingbloodproductionmechanismoffourformsofcobMtinsheepExperiment2：twentyrumencannulatedwetherswererandomlydividedintofivegroupswhichwerefedwiththebasaldietcontaining0086mgCo／kgDM(thecontr01)，thebasaldi

6、etsupplied，respectivelywith025，050，O75and100mgCo／kgDMasreagentgradeCOS047H20for8weeksControllambsshowedthemarginallevelofplasmavitaminBi2Supplementationof025to100mgCo／kgDMgaverisetoaquadraticincreaseinruminalandplasmavit

7、aminB12concentrationsandaquadraticdecreaseinplasmamethylmalonicacidconcentration(MMA)妒005)TheO50mgCo／kgDMseemstobeoptimumlevelforenhancedplasmavitaminB12andloweredMMAPlasmahomocysteine(HCY)wassignificantlydepressedbyCosu

8、pplementation(P005)with025mgsupplementalCo／kgDMenoughtosustainlowplasmaHCYlevelinlambsLambssupplementedwithO50mgCo／kgDMseemedtobebeneficialforproductionofrulnenTVFAandmolarproportionofpropionicandnutrientutilizationHowev

9、ersupplementalO25mgCo／kgDMisenoughtomeettherequirementforbloodproductionExperiment3：Twentylambswereallocatedrandomlytofivegroupsandfedwiththecontroldietwhichwassupplied03mgCo／kgDMtothebasaldiet，andfoursupplementaldietswh

10、ichweresuppliedeither03or06mg／kgDMofcobaltand10or20mg／kgDMofcopper(03：10，03：20，06：10and06：20)tothebasaldietrespectivelyVitaminB12productionbyrumenmicroorganismwasmainlyaccountedforbycobaltintakeSupplementationofcobaltand

11、copperincombinationwithdifferentlevelshasnocollaborateeffectsonvitaminB12synthesisandthedigestibilityofDM，OM，CPandNFE，wheasethedigestibilityofEE，NDFandADFwasincreasedandalsoironabsorptionandmetabolismwasenhanced，thusshee

12、pbloodproductionwasevidentlyimproved伊005)Experiment4：sixteenlambswereassignedrandomlytofourtreatmentgroups：Thecontrolgroupwhichwassupplementedwith030mgCo／kgDMtothebasaldiet，andotherfoursupplementalgroupswhichsupplied50，1

13、00and150mgZn／kgDMtothecontroldietrespectivelySupplemental50andl00mgZn／kgDMtothecontroldiethadnosignificantinfluenceonvitaminB12statusbutSupplemental150mgZn／kgDMresultedindecreaseinplasmavitaminBi2concentrationsandincreas