IBV感染雛雞巨噬細(xì)胞與抗體的相關(guān)免疫學(xué)變化.pdf

1、東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文IBV感染雛雞巨噬細(xì)胞與抗體的相關(guān)免疫學(xué)變化姓名：祝玉卿申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：預(yù)防獸醫(yī)學(xué)指導(dǎo)教師：魏萍20060620東北農(nóng)業(yè)大學(xué)農(nóng)學(xué)碩十論文TheDynamicChangesofImmunologyaboutMacrophageandAntibodyinSPFChickensInfectedwithInfectiousBronchitisVirusAbstractTheexperimentsucceedind

2、etectingspecificantibodyandgeneralantibodymselalmteartrachealandDuodenalhngataonofchickensIBV—infectedfrom1“dayto28“dayandthecontrolgroupbyindirectELISAwhichisfast，sensitive，beuerspecificityandrepeatabllityMeanwhile，011t

3、hebasisofs侄ongadhesioncapabilityandquickadherence，theexperimentsucceedinpenfyingmacrophagesbydifferentspeedadhesion，andinobtainingsplenicmacrophageandmedullarymacrophageofchickensbyregulatingadhesionculturaltimeandtemper

4、ature，thenindetectingtheactivityofmacrophagesbyassayingphagocyticcapacitytoneutralred，MTTeolorimetricassayassayingquantificationofAcPNOandIL一1Theresultsindmatethat：1IBVspeelficantibodycouldbedetectedonthe3rddayinSPFchike

5、nsinfectedIBVandtoppedon恤e14拍。andthelevelofantiIBVantibodykeptseveralweeks；thelocalimmunityemergedearlierthanthatofspecificinlnluueresponse，thelevelofnonspeeificantibodywaslowerthanthatincontrolgroupItshowsthatthetotalhu

6、moralimmunitydegraded，butthespecifichumoralimmunityenhanced，whiletheregionhumoralimmunityemergedfasterbuttransent，thehumoralimmunityofthewholebodyemergedslowlybutpersistencetimelonger2TheactivityofmaerophageinSPFchickens

7、infectedIBVWasclearlyhigherthanthatofthecontrolgroupInsplenicmacrophagethissituationhappenedbetweenthe7“dayandthe10tbday；whileinmedullarymacrophagethissituationhappenedbetweenthe3Iddayandthe10曲day，the7thdayandthe21“dayAl

8、lthatshowedtheinfectionofIBVwouldfurthertheactivationofmacrophage，enhancedthecapabilityofphagocytosis，thequanityofACPmcellsincreased，theactivityofsecretionandthecapabilityoftheatigenpresentaUonwerebothenhanced，andtookpar

9、tjnthemainprogressofinfectionThatWaSbasicallys鋤etothevantationofthehumoralimmunity，butitcmlastlongerthanthespecificalIgG3DunngtheinfectionofIBv’thequanityofILlincreasedinmaerophagewhichshowedimmunefunctionofmacrophagcWas

10、improvedAftertheinfectionofIBV，theeffectofmacrophageWassignificantThroughcytokmenetworkandtheeffectofantigenpresentation，maerophageassociatedwithotherimnlunecellsandimprovedspeccificinlnlBneresponseofSPFchickensThathints