貴金屬離子與蛋白質(zhì)的相互作用研究.pdf

1、蘭州大學碩士學位論文貴金屬離子與蛋白質(zhì)的相互作用研究姓名：黃艷申請學位級別：碩士專業(yè)：分析化學指導教師：常?？?0050501AbstractInthispapertheinteractionsofgammaseroglobulinumhumanum(GSH)，bovineserumalbumin(BSA)andbovinehemoglobin(Hb)withnoblemetalions，suchasIr(IV)，Pd(II)，Ru(I

2、I[)，Au(HI)ate，havebeeninvestigatedbyusingabsorptionspectra，fluorescencespectroscopysynchronousfluorescencespectroscopycirculardichroism(CD)spectroscopyandfouriertransforminfrared(FTIR)spectroscopyInthesystemofaceticacida

3、cetatesodiumbuffer(01molL～，pH5O)noblemetalionsmadetheintensityofthecharacteristicabsorptionpeakofproteinsenhance，accompaniedwithredshift，showingthatbindingofnoblemetalionstoproteinshadstrongimpactonproteinsconformationwi

4、thdecreaseofahelicalcontentofproteins；moreovernoblemetalionspossiblyreplacedFe(II)intheironporphyrinofHbTheintrinsicfluorescenceintensitiesofproteinswasquenchedwhennoblemetalionswereadded，andthequenchingmechanismofprotei

5、nsaffectedbynoblemetalionsWaSastaticquenchingprocedure，andthebindingnumber”andbindingconstantKwerecalculatedTheeffectsofnoblemetalionsonthesecondarystructureofproteinswerestudiedbycirculardichroismResultsshowedthatnoblem

6、etalionsinducedtheUhelicalcontentofproteinsdecrease，whichwasinagreementwiththatbyabsorptionspectraResultsofsynchronousfluorescencespectroscopyindicatedtheperturbationaroundthetryptophanresiduesInaddition，thequantitatives

7、econdarystructureconformationalanalysis，basedonfouriertransforminfrared(FTIR)selfdeconvolutionandcurve—fittingprocedures，ofamideIbandregion(17001600cm一1)ofHbandnoblemetalcomplexesinsolidstatewereinvestigatedNoblemetalion

8、sinducedahelicalcontentofproteinsdecrease397％or414％andincreased13sheetcontentofproteinsfrom52％to171％or159％Inaword，theinteractionbetweennoblemetalionsandproteinshadstrongimpactonproteinsconformationwithdecreaseofahelicalc