鼻腔鼻竇炎對(duì)嗅感覺神經(jīng)元凋亡影響的實(shí)驗(yàn)研究.pdf

1、復(fù)旦大學(xué)碩士學(xué)位論文鼻腔鼻竇炎對(duì)嗅感覺神經(jīng)元凋亡影響的實(shí)驗(yàn)研究姓名：胡松群申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：耳鼻咽喉科學(xué)指導(dǎo)教師：張重華20030510PartI：AbstractStudiesofamousemodelofacutebacterialrhinosinusitisObjective：TodevelopamousemodelofacutebacterialrhinosinusifisMethodsKMmicewereinoculat

2、edintranasallywithencapsuledStreptococcuspneumoniaetype3ThecontrolswereinoculatedwithsterilizedsalineThehistologicalsectionsofthenasalcavitiesandsinuseswerestainedwithhematoxiylin—eosinNeutrophilclustersinthenasalcavitie

3、sandsinuseswerecheckedResultsTherearesignificantredbloodceilsexudationonthe2ndand5thdayafterinoculationThenontheday8，Neutrophilclustersappeagandbyday11and14thenumberofNeutrophilclustersgainsitspeakTheextentofinflammation

4、decreasesonday28Conclusions：StreptococcuspneumoniaeinducesacutebacterialrhinosinusitisinKMmicewhichcanbeusedasananimaImodelofacutebacterialrhinosinusitisKeywords：acutebacterialrhinosinusitis，diseasemodel；animal，Streptoco

5、ccuspneumoniaePartII：ExperimentalstudiesonapoptosisofolfactoryreceptorneuronswithmiceacuterhinosinusitisObjective：ToobservetheapoptoticphenomenoninthemiceolfactoryepitheliumwithexperimentalrhinosinusitisMethods：Afterther

6、hinosinusitismodelshadbeenestablished，animals(4forsectionand2fortransmissionelectronmicroscopyeachtime)werebenevolentlysacrificedonday2，5，8，11，14，21，and28Thecontrols(4forsectionand2fortransmissionelectronmicroscopy)werek

7、illedonday8OlfactoryepitheliumwasobtainedfromtheolfactorycleftunderanatomicmicroscopeThefirstandthelastsectionsofeachanimal’Sweresampledtodetect0MPwithimmunofluorescencetechnique(1abeledbyRhodamine)Thethirdsectionswereus