腦缺血后細胞凋亡與PARP和Calpain的關系和意義.pdf

1、青島大學碩士學位論文腦缺血后細胞凋亡與PARP和Calpain的關系和意義姓名：李紅偉申請學位級別：碩士專業(yè)：人體解剖學指導教師：王守彪20030301英文摘要FUNCTONANDRELATIoNBETWEENA_PoPTOSISANDPAR_PoRCALPAINAFTERCEREBRAIISCHEM【IAABSTRACTAimThestudyistoexploretheexpressionofpoly(ADPribose)polyme

2、rase(PARP)andCalpaininfocalcerebralisehemiawithreperfusioninjuryandobservemorphologicalfeaturesofapoptoticcellsandtheroleofPARPandCalpalninapoptosisMethods36adultfemaleWistarratsweresubjectedtomiddlecerebralarteryocclusi

3、on(MCA01anddifferenttimeofreperfusionRatswererandomlydividedintoMCAOgroupsandshamgroupthatweresame∞operation，butnoMCAOToexploretheimplicationandthekineticsofsucheventinapoptosisinducedinsultinducedbyMCAOinsituTUNELtechni

4、cToobservetheexpressionofPARPandCalpainatthedifferenttimepointsandthenassessthcirfunctioninapoptosisinducedbyMCAOinsituhybridizationtechnicResultsApoptoticcellslieintheboundaryzonetotheischemiccoreThenumberofapoptoticcel

5、lsintheischemicCOreWaslessthanischemicpenumbra(p005)exceptfor2h，7d，14dSituhybridizationdemonstratedtheevidentexpressionofPARPat2hThepeakofPARPwasfirstdetectedat2hinthecorpusstriatumandthencerebralCO^eKat6hWithincreasingl

6、engthofsurvival，theexpressionofPARPbegantodecrease，thendroppedaverylowlevelat14dButtherealedifferencebetweenMCAOgroupandshamcontrol(p005)SituhybridizationdemonstratedtheevidentexpressionofCalpaininthecerebralcortexandcor

7、pusstriatumat2hThestudywasdetectedabimodalpaernofCalpainwithaninitialincreaseat2hfollowedbyamoreprominentsecondaryincreaseat12ha氕erreperfmionAfter24hCalpainbegan10decreasethendropped8verylowlevelat14dButtherearedifferenc

8、ebetweenMCAOgroupandshamcontrol(p005)ConclusionApoptosislieintheboundaryzonetotheischemiccore隊FuPandCalpainplayanimportantroleinapoptosisTheinhibitionofPARPandCalpaincanpreventandcurecerebraliscbemicdamage，Keywords：cereb