c-met siRNA抑制HepG2細(xì)胞c-met表達(dá)及誘導(dǎo)凋亡研究.pdf

1、浙江大學(xué)博士學(xué)位論文cmetsiRNA抑制HepG2細(xì)胞cmet表達(dá)及誘導(dǎo)凋亡研究姓名：樓海舟申請學(xué)位級別：博士專業(yè)：腫瘤學(xué)指導(dǎo)教師：吳金民20060401浙江大學(xué)博士學(xué)位論文IAPinhibitorsofapotosisproteins凋亡抑鋁0蛋白cOX一2cycl00xygenase一2環(huán)氧化酶一2HSF一1heatshockfactor1熱休克因子一1RHDRelhomologydolllain同源結(jié)構(gòu)域TSGtumorsupp

2、ressorgene腫瘤抑制基因MsGmetastasissuppressorgene轉(zhuǎn)移抑制基因刪P一9腿trixmetalloproteinase一9基質(zhì)金屬蛋白酶一9TIMPtissueinhibitormetalloproteinase組織型金屬蛋白酶抑制劑uPAurokinaseplasminogenactivator尿激酶纖溶酶原活化因子uPARurokinaseplasminogenactivatorreceptor尿激酶

3、纖溶酶原活化因子受體VEGFvascularendothelialgrowthfactor血管內(nèi)皮生長因子bFGFbasicfibroblastgrowthfactor堿性成纖維細(xì)胞生長因子踟baseInentmembrane基底膜EcMextracellularmatrix細(xì)胞外基質(zhì)EMTepithelialmesenchymltransition上皮一間質(zhì)轉(zhuǎn)化DFSdisease—freesurvivalrate無病生存率IHCi岫

4、unohistochemist珂免疫組化cFRc010nyformingrate克隆形成率DMS0dimethylsulfoxide二甲基亞砜PMSFphenylmethylsulfonylfluoride苯甲基磺酰氟dNTPsdeoxyribonucleosidetriphos曲ate脫氧核苷三磷酸DTTdithiothureit01二硫蘇糖醇EBethidiumbroⅢide溴化乙錠EDTAdisodiumethylenedi鋤in

5、etetra—acetate乙二胺四乙酸二鈉HEPESN一2一hydroxyethyipiperazine—N’一2ethanesulfonicacidN一2一羥乙基呱嗪一N’一2一乙磺酸sDSsodiumdodecylsulfate十二烷基硫酸鈉MTT：3一(4，5一dimethylthiaz01—2一y1)一2，5一dipherⅡyltetra—zoliumBromide四甲基偶氮唑藍(lán)PBS：phosphatebufferedsal