豚鼠單側(cè)迷路切除后前庭內(nèi)側(cè)核c-fos表達(dá).pdf

1、上海第二醫(yī)科大學(xué)碩士學(xué)位論文豚鼠單側(cè)迷路切除后前庭內(nèi)側(cè)核cfos表達(dá)姓名：顧美珍申請學(xué)位級別：碩士專業(yè)：耳鼻咽喉科學(xué)指導(dǎo)教師：殷善開20040401豚鼠單側(cè)謎路切除后前庭內(nèi)側(cè)核cfos表達(dá)ExpressionofcfosinmedialvestibularnucleusfollowingunilaterallabyrinthectomyinguineapigsAbstractObjective：Toinvestigatethec—fos

2、expressioninguineapigs’medialvestibularneuleus(MVN)followingunilaterallabyrinthectomy(UL)。Methods：Twenty—sevenguineapigswererandomlydividedintoninegroups，threeineachgroupAfterunilateral(right)labyrinthectomytheimmunohist

3、0109icaJstainingofcfoswasperformedFos—likeimmunoreactivity(FLI)wasanalyzedatfifteenminutesandlh、2h、6h、8h、12h、24h、2dand7dafterULResults：ResultsshowednoFosexpressioninthecontrolgroupBycontrast，F(xiàn)oswasconsistentlyinducedinth

4、eULguineapigsAsymmetricallabelingwasfoundinthebilateralmedialvestibularnucleus，withmoreFoslikeneuronsintheipsilateralMVNofthelesionedsidethaninthecontralateralMVN(P005)Thereactionbecameevident15minutespostULandwasintense

5、d12hoursafterthelesionFosexpressionpeakedearly(2h)anddeclinedprogressivelyHowever，weobservedasecondpeakofFosexpression(24h)，whichwasnotsoevidentasbeforeWestillfoundFosexpression7daysaftersurgeryConclusion：1)、ULcaninducer

6、eactivestrongFosimmunostainingintheMVN，whichisoneoftheimportantwayinwhichvestibularneuronsystemreactstooutsidestimulationBycomparingthesedatatothebehavioralrecoveryprocess，weconcludethattheearlyFosLIR(1～6h)likelyreflects

7、theactivationofneuralpathwaysinresponsetoULwhereasthedelayedFosLIR(24～48h)mightunderlinelongtermplasticchangesinvolvedintherecoveryprocess21、Thevestibularfunctionhasthegreatabilityofcompensation；3)、Theessenceofcfosandoth