磷(膦)酸肌醇化合物庫的篩選及生物學功能的研究.pdf

1、南開大學碩士學位論文磷（膦）酸肌醇化合物庫的篩選及生物學功能的研究姓名：張晶申請學位級別：碩士專業(yè)：生物化學與分子生物學指導教師：李魯遠201205AbstractLun2cancerisoneofthemmncausesofcancerrelateddeathintheworldAbout7585％oflungcanceriscomposedofNonsmallcelllungcancer(NSCLC)Peoplehavemadeal

2、otofprogressesincancerdiagnosis，chemotherapyandradiotherapybutthepatientsofNSCLCmayshowresistancetoradiotherapyandchemotherapyinthesurgicaltreatmentoflatestageTheoverall5yearsurvivalrateofDatientsofterminalstagesofcancer

3、afteroperationremainslessthan10％WeneedtofindnewmethodsteducetheincidenceP13K／AktpathwaywasfoundtobeimportanttoNSCLC，whictlCallbethetherapeutictargetforanticancerdrugsInosit01DhosphatecompoundscanbedividedintoPhosphoinosi

4、tide(IP)andPhosphatidylinositolphosphate(PtlnsP)，andplayanimportantroleincellsignaltransductionassignaling：moleculesXiprofessor’Steamhavesynthesizedsomeinositolcompoundswhichcanimprovethecellpenetrating，phosphorothioatea

5、ndinositolcompoundsofphosphoInicacidtoimprovethestabilityofthephospholipase，andfinallybuildthephosp]hoinositidephosphonicacidinositolanalogⅣescompoundlibrarytogetherwithsomeWedosomescreeningresearchusingA549cell，includin

6、gprescreeningwiththeconcentrationoflOItg／mLofthecompoundsandanalysisoftheconcentrationgradientfor14compoundswithbetterinhibiteffect，anddeterminetheirIC50Combinationofthe14compoundsandCisplatinwasinvestigatedtooWealsostud

7、ytheireffectonMCF10Aandother6cancercelllines，suchasMCF7Usingwesternblottingwedosomeresem：chtodeterminetheireffecttoP13K，Akt，Erkandotherkinases0809，0812，0414一MnwereinjectedtoC57micetodetectwhethertheycaninhibitthegrowthof