脊髓NR2B基因沉默對小鼠骨癌痛的影響.pdf

1、中南大學碩士學位論文脊髓NR2B基因沉默對小鼠骨癌痛的影響姓名：孫永軍申請學位級別：碩士專業(yè)：麻醉學指導教師：黃東20100501ABSTRACTobjective：Inordertoinvestigatethemechanismsofbonecancerpare，amousebonecancerpainmodeliSestablishedtostudytheeffectsofNR2Bgenesilencingbyintrathecal

2、injectionofPEI／NR2BsiRNAcomplexInthebonecancermouseToexplorethefeasibilityofcancerpaintreatmentbyusingNR2B／SiRNAtointerferetheexpressionofNR2BgeneinthevivoMethod：72adultmaleC57BL／6micewererandomlydividedintothreegroups：c

3、ancerpaingroup(N_24)：Lewislungcancercells2106／lOulwereinoculatedasstatedabove；interferencegroupaN=24)：PEI／NR2BsiRNAcomplexwasintrathecallyinjected12dayslateraftercancereellinoculation；controlgroupoftheinterference㈣4)：the

4、PEI／NegativecontrolsiRNAcomplexwasusedinintrathecalinjectionSpontaneousliftingtimeandmechanicalallodyniaweremeasuredpre—operationandonthe6m，10m，12m，13m，14n1，15m，16m，18血and22nddayafterinoculationL4—6spinalcordsegmentswere

5、isolatedforfluorescencequantitativeRTPCRonthe13山，14山，15m，16m，18ndand221mdayafterinoculationAccordingtotheresultsofthePCRtodoWesternBlottodetecttheexpressionlevelsofNR2BResults(1)Real—timequantitativePCRdetection：theexpre

6、ssionofNR2BinL4—6spinalcordintheinterferencegroupwasreducedsignificantlyascomparedtothecancerpaingroupandthecontrolgroupoftheinterferencegrouponthe14mandl8凼dayafterinoculation(P005)(2)TheresultsofWesternBlot：theexpressio