棗樹上類病毒種類鑒定及序列分析.pdf

1、福建農(nóng)林大學(xué)碩士學(xué)位論文棗樹上類病毒種類鑒定及序列分析姓名：劉光要申請學(xué)位級別：碩士專業(yè)：植物病理學(xué)指導(dǎo)教師：王宗華李世訪20080401AbstractNinetyfiveJujubetreesampleswerecollectedfromCangzhouBaodingandHandaninHebeiprovinceofChinaThesesampleswereusedfordetectionandsequencediversitya

2、nalysisofviroidsLowmolecularweightRNAswereextractedandusedforDotblotHybridization，ReverseTranscriptionPolymeraseChainReaction(RTPCR)，TwoDimensionalPAGE(2DPAGE)andTransferMembraneforHybridizationandBiologicalIndexingResul

3、tsshowedthatthe2Jujubetreesampleswhichwerecollectedfromthesamefieldof“Jujubetree—pear’intercropinginQiqiao，CangzhouwerepositiveforHopstuntviroid(HSVd)TheothertwoareaswereallnotdetectedHSVdinJujubetreesThedetectionratewas

4、21％ThisisthefastreportofHSVdinJujubetreesintheworldBothofthe2sampleswereselectedforcloning，sequenceanalysisofHSVdandthencomparingwiththefastHSVdsequence(X00009，1983)fromtheHopandalsotheHSVdsequencesinGenBankResuItsindica

5、tedthat：1Eachof2HSVdsequenceswereclonedfromCZ3andCZ4，thencamparedwiththeHSVdsequence(xoo009，1983)whichwasfirstreportfromtheHop，theresultsshowedthat25genesiteshadbeenmutatedtheyhadlowhomology2TheHSVdsequencesinJujubetreef

6、romChinawererelativelyconservedandeachoftheHSVdsequenceBlastinGenBankshowedthathomologyswerefrom92％to100％，andtherewere110clearrelativityamongsequencevariationregionandhostKeywords：Jujubetree，HopstuntviroidDetectiontechno