不同低溫對(duì)鼠永久性大腦中動(dòng)脈閉塞后NF-кB表達(dá)及腦梗死體積的影響.pdf

1、三峽大學(xué)碩士學(xué)位論文不同低溫對(duì)鼠永久性大腦中動(dòng)脈閉塞后NFкB表達(dá)及腦梗死體積的影響姓名：蘭晶申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：免疫學(xué)指導(dǎo)教師：李耀彩20070401IIIAbstractObjectiveThroughinvestigatingtheeffectofdifferenthypothermiaonexpressionofNFκBinneuronofinfarctregiontheinfarctionvolumefollowingpe

2、rmanentdlecerebralarteryocclusion(MCAO)inratsresearchmechanismofhypothermicneuroprotection.Methods120maleSpragueDawleyratsweredividedromlyinto3groups:nmothermiagroup(37℃)mildhypothermiagroup(34℃)moderatehypothermiagroup(

3、32℃)everygroupweredivided4subsets:ischemia2h4h6h12h.Eachsubsethad10rats.Permanentdlecerebralarteryocclusionmodelswereestablishedbyusingtheintraluminalsuturemethod.Theratswereplacedonconspleiceblanketmachineafterischemia2

4、h4h6h12hrespectively.Meanwhiletherectaltemperatureweremaintained370.5℃，340.5℃320.5℃12hrespectively.Theratsweresacrificedinpretimepoint.3ratsineverysubsetswereusedinmeasuringinfarctionvolumewithTTCstaining.Theotherswereus

5、edinmeasuringtheexpressionofNFκBwithimmunohistochemicalstaining.ResultAt2h4h6h12hofpostischemiainnmothermicgrouptheinfarctvolumewere1.836.38㎜56.073.28㎜176.2810.10㎜338.2229.66㎜respectively.At2h4h6h12hofpostischemiainmildh

6、ypothermiagrouptheinfarctvolumewere22.2211.36㎜45.892.75㎜108.2942.44㎜255.0727.57㎜respectively.At2h4h6h12hofpostischemiainmoderatehypothermiagrouptheinfarctvolumewere16.866.80㎜37.692.95㎜68.957.72㎜209.9915.41㎜respectively.C

7、omparedtonmothermicgroupexceptingafterischemia6hofmildhypothermiagrouptheinfarctvolumeofmildhypothermiagroupmoderatehypothermiagroupweresignificantlydecreased（P﹤0.05&P﹤0.01）ineachischemiatimepoint.At2h4h6h12hofpostischem

8、iainnmothermicgroupthevalueofaverageintegralopticaldensity(aIOD)was62.0316.0771.2124.45107.0933.08107.0554.09respectively.At2h4h6h12hofpostischemiainmildhypothermiagroupthevalueofaIODwas37.4512.1959.0220.9152.1219.4948.1