玉米大斑病菌鈣調(diào)磷酸酶B亞基基因的克隆與功能分析.pdf

1、河北農(nóng)業(yè)大學(xué)碩士學(xué)位論文玉米大斑病菌鈣調(diào)磷酸酶B亞基基因的克隆與功能分析姓名：劉艷梅申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：植物學(xué)指導(dǎo)教師：韓建民董金皋20080606CloningandFunctionalAnalysisoftheCalcineurinBGeneinSetosphaeriaturcicaAuthorLIUYahrodSupervisors：HANJianmin，DONGJingaoMajorBotanyAbstractCalcine

2、ufinisaconservedCa2／calmodulinactivatedserine／threoninespecificprotainphosphatasethatplaysallimportantroleintheregulaticnofmorphogenesisandpathogenesisinvariousfungiInthisworkCnBgenewasclonedandidentifiedfromSetosphaeria

3、turcica，thenstudiedanddeduceditsrolesin&turcicadevelopmentbyadvantageofantisenseinhibitionexpressionmethodandbioinformaticsanalysisItisthefirsttimeforUStoisolatethecompletedeDNAsequenceandDNAsequenceofCnBgenefrom&turcica

4、llsingRACEmethod，andpredictthecorrespondingstructureandfunctionsbythebioinformaticsoftwareTheresultsshowedthatCnBgenehad4exonsand3intronsandthelargestopenreadingframeWas525bpwhichencodedaproteinof174aminoacidresiduesTheC

5、nBgeneof&turcicahadfour“EFhand’calciumbindingregions，andshowed90％homologywimCnBgenesfrom只nodorum，BcinereaandNeurosporacrassaPredicted3DstructureforCnBproteinbySwissmodel，wascomtimtedwith8helixesand4turns，forming4‘helixes

6、loophelixes’’regionsThestructurecharacteristicwasconsistentwimCnBfour“EFhand’calciumbindingregionsA2kbgeneflankregionWasobtainedbyGenomicWalkingBioinformaticanalysisshowedthatithadalotofcis—actingdementscorrespondingGbox

7、，DETl，CDAI，HSF，butnotypicalTATAboxWecalculatedthatCnBgeneperhapsplaysanimportantroleintheregulationofcellmorphogenesis，hottoleranceandlightsignalconductionTheresultofSouthemblottingshowedthatCnBWassinglecopyinthegenomeof

8、SturcicaThesenseorantisenseCnBgeneexpressioninductionvectorswereconstructedbasedontheplasmidpSOIthentransformedinto&turcicaprotoplastand5antisenseCnBgenewhichhphgeneinsertedwerescreenedfrom23transformantsTwotransformants