戊四氮點(diǎn)燃前后大鼠海馬區(qū)神經(jīng)元線粒體結(jié)構(gòu)和功能的動(dòng)態(tài)變化.pdf

1、分類號(hào)UDC密級(jí)編號(hào)豐I豹大學(xué)CENTRALSoUTHUNIVERSITY碩士學(xué)位論文論文題目：學(xué)科、專業(yè)：研究生：導(dǎo)師姓名及其專業(yè)技術(shù)職稱：戊四氨點(diǎn)燃前后大鼠海馬區(qū)神經(jīng)元線粒體結(jié)構(gòu)和功能的動(dòng)態(tài)變化神經(jīng)病學(xué)周艷輝肖波教授中南大學(xué)二oo七年五月碩士學(xué)位論文英文摘要Changesofmitochondrialultramicrostrctureandfunctionbeforeandafterpentetrazolekindlinginth

2、erat’shippocampusABSTRACTobjectToobservethechangesofmitochondrialultramicrostrctureandfunctionintherat’shippocampnsbeforandafterpentetrazole(PTZ)inducedseizuressuccessfullyandtoinvestigatetheroleofmitochondrialdisfunctio

3、ninepileptogenisisMethods60healthymaleSpragueDawleyratsweredividedrandomlyintocontrolandPTZtreatedgroupsThelatterwereintraperitoneallyinjectedPTZ(30mg／kg)every24hoursnlecontrolgroupsreceived09％salineinjectionsinsteadofPT

4、ZatthesanletimeSeizuremanifestationswereevaluatedaccordingtoRacineandEEGWhentheyreachedeachstandard(Igrade，IIIgrade，kindled，afterkindled24hoursandafterkindled72hours)，theywouldbeusedtoobservethemitochondrialultramicrostr

5、ctureandtodetectits’functionsrespectivelyResults1AnimalmodelAninitialsubconvulsivedoseofPTz00mg／kg)didnotelicitamotorresponseSubsequentinjectionwiththesamedoseinducedconvulsiveresponseofgraduallyincreasingseverityEachgro

6、upneedtimeasfollows：IgradeWas7釷17days，IIIgradewas170士69days，kindledwas25159days，afterkindled24hourswas27Oa：54daysafterkindled72hourswas28155days967％animalsreachedeachstandardsuccessfullyEpilepticformdischargeswerefoundin

7、eachPTZgroupAllthekindledratshadspontaneousseizureswhilethecontrolgrouphadneitherseizuresnorabnormaldischargesontheEEG2ThechangesofmitochondrialultramicrostrctureWecouldobservethatthemltochondfiaofthecon仃olwereintegrityT